PMID: 489987Jan 1, 1979Paper

Separation of human lymphocytes and monocytes by velocity sedimentation at unit gravity

Journal of Immunological Methods
W S BontH A Loos

Abstract

An improved method of separating human peripheral blood lymphocytes and monocytes by velocity sedimentation at unit gravity is described. Cell separation capacity is considerably increased, while the time required for optimal separation is markedly reduced. Simple devices make it possible to layer and fractionate the gradients within 15 min. Overall recovery is 85%. On a small scale, monocytes were isolated greater than 80% pure from 4--20 X 10(7) mononuclear leukocytes. The average yield was 40% of all monocytes recovered after fractionation. In the same sedimentation runs 60% of all recovered lymphocytes were obtained strongly depleted of monocytes (monocyte contamination less than 0.2%). On a large scale 3--6 X 10(8) monocytes with an average purity of 85% were isolated from 3--6 X 10(9) mononuclear leukocytes in 2 subsequent runs. All cells recovered from the gradient were functionally active according to rosette formation, phagocytosis, stimulation by mitogens and in cytotoxicity tests.

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Citations

Jan 1, 1980·Journal of Immunological Methods·A J TrevesZ Fuks
Jun 24, 1983·Journal of Immunological Methods·R A De WegerW Den Otter
May 13, 1983·Journal of Immunological Methods·U Kärnström, T Hallberg
May 15, 1985·Thrombosis Research·F BellocJ Soria
May 13, 1998·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J A Loos, J L Wautier
Nov 9, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·De-En HuKevin M Brindle
Oct 28, 1983·Journal of Immunological Methods·C B ThompsonI Scher
Apr 1, 1984·Seminars in Nuclear Medicine·J G McAfeeG Gagne
Sep 1, 1980·Analytical Biochemistry·A TulpM G Barnhoorn
Jan 1, 1986·Critical Reviews in Clinical Laboratory Sciences·A G Desai, M L Thakur
Mar 1, 1980·Experimental Cell Research·J G CollardJ Boezeman
Feb 15, 2013·Pharmacological Reports : PR·Bryan K Sanders
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jogchum Plat, Ronald P Mensink
Sep 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·G MüllerG Schmitz
May 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H GinsbergW V Brown
Nov 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M C TerritoA M Fogelman
Nov 14, 1998·The American Journal of Physiology·B D UhalM Selman

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