PMID: 7540498Apr 1, 1995Paper

Separation of lipoproteins, albumin and gamma-globulin by single-step ultracentrifugation of human serum. Application. I: Binding of hematoporphyrin to human serum and to albumin

The International Journal of Biochemistry & Cell Biology
M Kongshaug, J Moan

Abstract

Previous studies of the serum binding of the photosensitizer hematoporphyrin (Hp) have given widely different results. The serum binding of Hp is therefore further illuminated by experiment and discussion. Ultracentrifugal separation of serum is improved and applied to study the binding of Hp to human serum and HSA. The observed distribution of Hp among the serum proteins is compared with the distribution expected from available association constants for Hp binding with individual proteins. The lipoprotein classes and the two major high density proteins (HDP), albumin and gamma-globulin, were separated in a NaCl-KBr gradient by single spin ultracentrifugation (SW 40; 30,000 rpm). HSA- and HDP-bound Hp were similarly distributed in the centrifuged gradient. Centrifugation of Hp-doped HSA separated the unbound Hp (75%) and the HSA-bound Hp (25%). The present association constant for the Hp-HSA complex (10(3)/M) was much lower than earlier published ones (10(6)/M) found by other techniques. The association of Hp with HDP in serum was much stronger than the association of Hp with the isolated HSA (electrophoretic grade). The estimated ratio of HSA-bound to LDL-bound HP in serum was at least 25 times larger than the experimental val...Continue Reading

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Citations

Apr 19, 2002·Journal of Photochemistry and Photobiology. B, Biology·A K Haylett, J V Moore
Jan 24, 1998·Journal of Photochemistry and Photobiology. B, Biology·S SilA S Chakraborti
Jun 17, 2000·The International Journal of Biochemistry & Cell Biology·B CunderlíkováJ Moan
Dec 17, 2005·Antioxidants & Redox Signaling·U Julius, J Pietzsch
Dec 15, 2010·FEBS Letters·Alicja SznarkowskaJoanna Zawacka-Pankau
Oct 24, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Benoît ChauvinAthena Kasselouri

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