Separation of metanephrine and normetanephrine from urine for automated fluorimetric routine determination (author's transl)

Clinica Chimica Acta; International Journal of Clinical Chemistry
G Schwedt, H H Bussemas

Abstract

It is possible to separate metanephrine and normetanephrine from urine for the automated fluorimetric routine determination by means of a combination of liquid-liquid partition with ethylacetate and clean-up of the extract through Amberlite XAD-4. The differentiated fluorimetric determination is based on the oxidation with potassium hexacyanoferrate (III) in the presence of zinc ions and different pH values in an autoanalyzer. No quenching effects were observed. The recovery yields were in the range of 60%, the relative standard deviation being less than 10%.

References

Feb 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J VallonC Bichon
Oct 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·F Geissbuhler
Nov 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·L M BertaniS E Gitlow
Sep 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·Z Kahane, P Vestergaard
Apr 1, 1966·Clinica Chimica Acta; International Journal of Clinical Chemistry·R F Coward, P Smith
Jul 1, 1966·Clinica Chimica Acta; International Journal of Clinical Chemistry·G L MattokR A Heacock
Nov 1, 1966·Clinica Chimica Acta; International Journal of Clinical Chemistry·R F Coward, P Smith
Feb 1, 1961·The Journal of Clinical Investigation·E H LABROSSES S KETY
Nov 1, 1961·Clinica Chimica Acta; International Journal of Clinical Chemistry·O KRAUPPD PAPISTAS

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