Separation of multiphosphorylated cyclopeptides and their positional isomers by hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization mass spectrometry (ESI-MS).

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lana Abou ZeidCarole Bresson

Abstract

Peptides are efficient models used in different fields such as toxicology to study the interactions of several contaminants at the molecular scale, requiring the development of bio-analytical strategies. In this context, Hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization mass spectrometry (ESI-MS) was used to separate synthetic multiphosphorylated cyclopeptides and their positional isomers at physiological pH. We assessed (i) the selectivity of eleven HILIC columns, from different manufacturers and packed with diverse polar sorbents, and (ii) the effect of mobile phase composition on the separation selectivity. The best selectivity and baseline resolution were achieved with the columns grafted by neutral sorbents amide and diol. Furthermore, we investigated the HILIC retention mechanism of these peptides by examining the effect of the number of phosphorylated residues in the peptide scaffold on their retention. The peptide behavior followed the classical hydrophilic partitioning mechanism exclusively on amide and diol columns. This trend was not fully respected on bare and hybrid silica due to the attractive/repulsive interactions of the deprotonated surface silanol groups with the Arginine...Continue Reading

References

Apr 30, 2005·Inorganic Chemistry·Huan HuangJ David Van Horn
Sep 15, 2006·Journal of Separation Science·Petrus Hemström, Knut Irgum
Jan 16, 2007·Journal of Chromatography. a·Akio YanagidaYoichi Shibusawa
Jan 30, 2007·Journal of Proteome Research·Paul J BoersemaShabaz Mohammed
Dec 16, 2010·Journal of the American Chemical Society·Anaïs M PujolPascale Delangle
May 3, 2011·Journal of Chromatography. a·Martin Gilar, Aleksander Jaworski
Sep 1, 2011·Analytical and Bioanalytical Chemistry·Bogusław Buszewski, Sylwia Noga
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Matteo TegoniVincent L Pecoraro
Jul 5, 2013·Chemistry : a European Journal·Samir SafiChristophe Den Auwer
Mar 13, 2014·Annals of the New York Academy of Sciences·Christelle Gateau, Pascale Delangle
Oct 18, 2014·Chemistry : a European Journal·Colette LebrunPascale Delangle
Nov 22, 2014·Journal of Separation Science·Aurélie PeriatDavy Guillarme
Feb 7, 2017·Chemistry : a European Journal·Matthieu StarckPascale Delangle
Feb 25, 2017·Analytical Chemistry·Alba CristobalAlbert J R Heck
May 14, 2020·Metallomics : Integrated Biometal Science·Marie MonestierElisabeth Mintz

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