Separation of phenolic acids by capillary electrophoresis with indirect contactless conductometric detection

Electrophoresis
Petr KubánVlastimil Kubán

Abstract

A new method for the electrophoretic separation of nine phenolic acids (derivatives of benzoic and cinnamic acids) with contactless conductometric detection is presented. Based on theoretical calculations, in which the mobility of the electrolyte co- and counterions and mobility of analytes are taken into consideration, the electrolyte composition and detection mode was selected. This approach was found to be especially valuable for optimization of the electrolyte composition for the separation of analytes having medium mobility. Indirect conductometric detection mode was superior to the direct mode as predicted theoretically. The best performance was achieved with 150 mM 2-amino-2-methylpropanol electrolyte at pH 11.6. The separation was carried out in a counter-electroosmotic mode and completed in less than 6 min. The LODs achieved were about 2.3-3.3 microM and could be further improved to 0.12-0.17 microM by using a sample stacking procedure. The method compares well to the UV-Vis detection.

References

Aug 24, 1999·Journal of Chromatography. a·M U KatzmayrW Buchberger
Jul 15, 2003·Electrophoresis·Alexandre Zatkovskis CarvalhoClaudimir L do Lago
Mar 10, 2006·Journal of Separation Science·Dagmar SterbováVlastimil Kubán
Feb 1, 1998·Analytical Chemistry·A J ZemannG K Bonn

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Citations

Nov 9, 2012·Journal of Chromatographic Science·Violeta NourSina Cosmulescu
Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Elena Hurtado-FernándezAlberto Fernández-Gutiérrez
Feb 1, 2007·Electrophoresis·Stefan BachmannGünther K Bonn
Sep 15, 2006·Journal of Separation Science·Veronika Solínová, Václav Kasicka
Dec 28, 2006·Electrophoresis·Petr KubánVlastimil Kubán
Dec 7, 2007·Electrophoresis·Ewa Dabek-ZlotorzynskaMahmoud M Yassine
Dec 25, 2007·Analytica Chimica Acta·Pavel Kubán, Peter C Hauser
Nov 24, 2007·Journal of Chromatography. a·Steven L SimpsonShigeru Terabe
Dec 23, 2008·Electrophoresis·Zdena MaláPetr Bocek

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