PMID: 8590806Dec 1, 1995Paper

Separation of phenotypes in mutant alleles of the Schizosaccharomyces pombe cell-cycle checkpoint gene rad1+

Molecular Biology of the Cell
G Kanter-SmolerS Subramani

Abstract

The Schizosaccharomyces pombe rad1+ gene is involved in the G2 DNA damage cell-cycle checkpoint and in coupling mitosis to completed DNA replication. It is also required for viability when the cdc17 (DNA ligase) or wee1 proteins are inactivated. We have introduced mutations into the coding regions of rad1+ by site-directed mutagenesis. The effects of these mutations on the DNA damage and DNA replication checkpoints have been analyzed, as well as their associated phenotypes in a cdc17-K42 or a wee1-50 background. For all alleles, the resistance to radiation or hydroxyurea correlates well with the degree of functioning of checkpoint pathways activated by these treatments. One mutation, rad1-S3, completely abolishes the DNA replication checkpoint while partially retaining the DNA damage checkpoint. As single mutants, the rad1-S1, rad1-S2, rad1-S5, and rad1-S6 alleles have a wild-type phenotype with respect to radiation sensitivity and checkpoint functions; however, like the rad1 null allele, the rad1-S1 and rad1-S2 alleles exhibit synthetic lethality at the restrictive temperature with the cdc17-K42 or the wee1-50 mutation. The rad1-S5 and rad1-S6 alleles allow growth at higher temperatures in a cdc17-K42 or wee1-50 background tha...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1992·The EMBO Journal·F al-Khodairy, A M Carr
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G JimenezS Subramani
Nov 3, 1989·Science·L H Hartwell, T A Weinert
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Dec 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K S Sheldrick, A M Carr

❮ Previous
Next ❯

Citations

Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A R Lehmann
Sep 2, 1998·Trends in Genetics : TIG·P A JeggoA R Lehmann
Jan 23, 1999·Current Opinion in Cell Biology·N Rhind, P Russell
Dec 12, 2001·Molecular Biology of the Cell·R KaurT Enoch
Apr 4, 1998·Molecular Biology of the Cell·M DahlenP Sunnerhagen
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·O Cohen-Fix, D Koshland
Mar 3, 2004·Nature Reviews. Cancer·Bin-Bing S Zhou, Jiri Bartek
Jul 11, 1998·The Journal of Biological Chemistry·A E ParkerW H Luyten
Aug 5, 2003·Molecular and Cellular Biology·Michael N BoddyPaul Russell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.