Separation of polyethylene glycols and their amino-substituted derivatives by high-performance gel filtration chromatography at low ionic strength with refractive index detection

Journal of Chromatography. a
Min LiuWeiyue Lu

Abstract

The chromatographic analysis of polyethylene glycols (PEG) and their amino-substituted derivatives, with an average molecular mass of 2000 and 3350 is described. The PEG derivatives were perfectly separated by TSK-GEL G4000PW(XL) column, which was widely used in high-performance size-exclusion chromatography (SEC), at low ionic strength with refractive index detection. It was shown that ion-exchange interactions were mainly involved in the retention mechanism of these compounds on TSK-GEL G4000PW(XL) column, since retention volume decreased when salt concentration were added to the mobile phase and varied with the pH of the eluent. Additionally, size exclusion for PEG chains plays a role. Organic modifiers also had effect on chromatographic behaviors of PEG compounds.

References

Apr 14, 1995·Journal of Chromatography. a·T D BrumeanuC Bona
Jun 14, 2002·International Journal of Pharmaceutics·Tatiana S LevchenkoVladimir P Torchilin
Feb 5, 2003·Journal of Chromatography. a·Christina RappelAlexei Gorbunov
May 20, 2003·International Journal of Pharmaceutics·Hong LiKun Han

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Citations

Sep 14, 2007·International Journal of Pharmaceutics·Haizheng Zhao, Lin Yue Lanry Yung
Jun 26, 2007·Journal of Pharmaceutical and Biomedical Analysis·Virginia ZabaletaJuan M Irache
Jan 30, 2015·Journal of Separation Science·Ziping WeiPatricia W Cash

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