PMID: 9167935Apr 1, 1997Paper

Separation of representative lipid compounds of biological membranes and lipid derivatives from peroxidized polyunsaturated fatty acids by reversed phase high-performance liquid chromatography

Free Radical Research
D Di PierroB Giardina

Abstract

A complex mixture of different lipid compounds, including phosphatidylcholine, phosphatidylserine, all trans-retinol, 15(S)-hydroperoxyeicosatetraenoic acid, D-alpha-tocopherol, saturated and unsaturated fatty acids can be separated by reversed phase HPLC by using a C-18, 120 mm x 4 mm, 3 microns particle size column and a step gradient from acetonitrile/water (1:1; v:v) to 100% acetonitrile at a flow rate of 0.8 ml/min. By applying this elution condition, separation of various groups of lipid hydroperoxides and lipid derivatives, each one originating from a different in vitro peroxidized polyunsaturated fatty acid, can be obtained. Simultaneous detection is carried out by a diode array detector at a wavelength accumulation range set up between 195 and 400 nm. The possibility of simultaneously having such a large number of measurements renders this chromatographic method particularly suitable in studies concerning lipid peroxidation where, in addition to the detection of free radical-induced lipid hydroperoxides, data on some key antioxidant molecules, i.e. vitamin A and E, as well as that of structural compounds of biological membranes, i.e. phosphatidylcholine and phosphatidylserine, can be achieved.

References

Mar 1, 1992·Lipids·G Minotti, S D Aust
Oct 21, 1992·Molecular and Cellular Biochemistry·D Di PierroB Giardina
Jan 1, 1992·Free Radical Research Communications·C K Chow
Jan 1, 1992·Free Radical Research Communications·G LazzarinoB Giardina
Jan 1, 1992·Free Radical Biology & Medicine·P PalozzaN I Krinsky
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Apr 1, 1990·Archives of Biochemistry and Biophysics·D C Laudicina, L J Marnett
May 31, 1990·Biochemical and Biophysical Research Communications·J K BeckmanH L Greene
May 15, 1989·Archives of Biochemistry and Biophysics·D M Miller, S D Aust
Jan 17, 1985·The New England Journal of Medicine·J M McCord
Jan 1, 1985·Journal of Free Radicals in Biology & Medicine·A Sevanian, E Kim
Jan 1, 1993·Free Radical Research Communications·J M Gutteridge
Sep 1, 1993·The British Journal of Nutrition·D M WalshS Kennedy

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Citations

Jul 27, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J KaewsrithongT Ohshima

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