Sep 1, 1976

Separation of slow reacting substance of anaphylaxis (SRS-A) from human lung into four biologically active fractions

The Journal of Immunology : Official Journal of the American Association of Immunologists
H TakahashiH H Newball

Abstract

Slow reacting substance of anaphylaxis (SRS-A) was released from human lung passively sensitized with ragweed antibody and challenged with specific antigen E. After purification by ethanol extraction, incubation with alkali (0.1 M NaOH for 30 min at 37 degrees C) and chromatography on silicic acid and DEAE-cellulose, human SRS-A was separated into four biologically active fractions (Fractions I to IV). Arylsulfatase (Type H-1) in 0.1 M sodium acetate buffer, pH 4.5, destroyed the biologic activity of only Fraction I. All four fractions, like SO4=, inhibited the arylsulfatase activity at pH 4.5 but not at pH 6.0 when p-nitrocatechol sulfate was used as substrate. These results suggest that SRS-A contain a sulfur group and that human STS-A, like the prostaglandins, may be a family of compounds. The instability of the purified SRS-A to storage remains a major barrier to their further purification and chemical identification.

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Mentioned in this Paper

Ethanol
Ethanol Measurement
Lung
Chromatography
Sulfur
Effects of Radiation
Silicic Acid
DEAE-Cellulose
4-nitrocatechol sulfate
Specific antigen

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Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.