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Separation of specific antibody-forming mouse cells by their adherence to insolubilized endogenous hormones

The Journal of Clinical Investigation

Jan 1, 1974

Kenneth L MelmonS Bauminger

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Abstract

Spleen cells from mice immunized with sheep red cells were separated by differential adherence to insolubilized histamine, catecholamines, and prostaglandins. The hormones were insolubilized by linking them to Sepharose beads through a protein carrier. We measured hemolytic plaque forma...read more

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  • Separation of specific antibody-forming mouse cells by their adherence to insolubilized endogenous hormones

    The Journal of Clinical Investigation

    Jan 1, 1974

    Kenneth L MelmonS Bauminger

    PMID: 4357614

    DOI: 10.1172/jci107542

    Abstract

    Spleen cells from mice immunized with sheep red cells were separated by differential adherence to insolubilized histamine, catecholamines, and prostaglandins. The hormones were insolubilized by linking them to Sepharose beads through a protein carrier. We measured hemolytic plaque forma...read more

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