PMID: 2312Jan 15, 1976

Separation of subchloroplast membrane particles by counter-current distribution

Biochimica Et Biophysica Acta
B AnderssonP A Albertsson

Abstract

Counter-current distribution in an aqueous Dextran-polyethylene glycol two-phase system has been used to fractionate membrane fragments obtained by press treatment of Class II chloroplasts. By the counter-current distribution technique membrane particles are separated according to their surface properties such as charge and hydrophobicity. The fractions obtained were analysed with respect to photochemical activities, chlorophyll and P-700 contents. The Photosystem II enrichment after counter-current distribution was better than that obtained by differential centrifugation of the disrupted chloroplasts. However, the best separation of Photosystem I and II enriched particles could be achieved if differential centrifugation was combined with the counter-current distribution technique. Each centrifugal fraction could be further separated into Photosystems I and II enriched fractions since the Photosystem II particles preferred the dextran-rich bottom phase while the Photosystem I particles preferred the polyethylene glycol-rich top phase. By this procedure it was possible, without the use of detergents, to obtain vesicles which were more enriched in Photosystem II as compared to intact grana stacks. The partition behaviour of undis...Continue Reading

References

Aug 4, 1970·Biochimica Et Biophysica Acta·P V SaneR B Park

Citations

Feb 1, 1996·Photosynthesis Research·Inger Carlberg, B Andersson
Jan 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Inger CarlbergA V Vener
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·C SpeteaB Andersson
Jun 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·U Rosing, A Olund
Apr 1, 1982·European Journal of Biochemistry·B AnderssonI J Ryrie
Jul 21, 1995·The Journal of Biological Chemistry·K D IrrgangW P Schröder
May 23, 1991·European Journal of Biochemistry·A M PramanikB Andersson

Related Concepts

Cell Fractionation
Chlorophyll
Etioplasts
Countercurrent Chromatography
Tissue Membrane
Electron Microscopy
NADP
Sodium 2,6-Dichloroindophenol

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