Separation of the enantiomers of ibuprofen and its major phase I metabolites in urine using capillary electrophoresis

Electrophoresis
I BjørnsdottirS H Hansen

Abstract

A capillary electrophoresis method for determination of the enantiomers of ibuprofen and its major phase I metabolites: 2'-hydroxyibuprofen and 2'-carboxyibuprofen in urine samples have been developed. Cyclodextrins and linear dextrins have been investigated as chiral selectors. Simultaneous chiral separation of the enantiomers of ibuprofen, 2'-hydroxyibuprofen and 2'-carboxyibuprofen was obtained using a mixture of dextrin 10 and heptakis (2,3,6-tri-O-methyl)-beta-cyclodextrin in a 2-[N-morpholino]ethanesulphonic acid buffer, pH 5.26. The electroosmotic flow was reversed using hexadimethrine bromide as a buffer additive. The method can be used for the determination of the free enantiomers of ibuprofen, 2'-hydroxyibuprofen and 2'-carboxyibuprofen as well as for the indirect determination of their glucuronic acid conjugates in urine samples.

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Citations

Oct 6, 2004·Archives of Pharmacal Research·Man-Jeong Paik, Kyoung-Rae Kim
Jun 12, 2008·European Journal of Drug Metabolism and Pharmacokinetics·Chaonan ZhengJing Li
Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pierina Sueli BonatoRoberto de Carvalho
Jun 6, 2000·Journal of Chromatography. a·S Zaugg, W Thormann
Feb 28, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C SimóA Cifuentes
Apr 4, 2000·Journal of Pharmaceutical and Biomedical Analysis·C M BooneW J Underberg
May 24, 2006·Electrophoresis·Rukhsana JabeenJohn Petersen
Nov 30, 2005·Electrophoresis·Wang-Chou Sung, Shu-Hui Chen
Sep 26, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S C TanA J Hutt
Jun 2, 2009·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M Karaźniewicz-ŁadaF Główka
Jun 6, 2000·Journal of Chromatography. a·G Blaschke, B Chankvetadze

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