Jan 1, 1975

Separation of the hormones of the adenohypophysis of rats by use of electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate

Ukrainskiĭ biokhimicheskiĭ zhurnal
F P Martynenko, T A Sakhno

Abstract

A comparative study of rat adenohypophysis extract and its alcohol fractions was performed by two variants of the method of electrophoresis in polyacrylamide gel: at pH 9,5 and with the presence of sodium dodecyl sulphate at pH 7.2. With the presence of sodium dodecylsulphate four protein zones are found which in the order from the anode towards the cathode are identified as hemoglobin, somatotropin, lactotropin and albumin. It is shown that the somatotropin zone after the extract separation at pH 9.5 is inhomogeneous and consists of somatotropin and hemoglobin.

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Mentioned in this Paper

Pituitary Hormones, Anterior
Polyacrylamide Gels
SDS-PAGE
Hemoglobin Measurement
Pituitary Gland, Anterior
Alb
Growth Hormone Measurement
Irium
Sodium Dodecyl Sulfate
Growth Hormone

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