PMID: 6108213Sep 1, 1980Paper

Separation of the proteins of bovine milk-fat-globule membrane by electrofocusing with retention of enzymatic and immunological activity

European Journal of Biochemistry
I H MatherM G Irving

Abstract

1. Proteins of fat-globule membrane from bovine milk were solubilized with the non-ionic detergent Triton X-100 in the presence of protease inhibitors. Approximately 25% of the total membrane protein was solubilized and the extracts were shown to contain a sample of most of the major membrane proteins and glycoproteins. 2. The solubilized proteins were separated in flat-beds of Ultrodex by electrofocusing and the pI values for the major proteins, glycoproteins and certain enzymes determined. Several of the proteins displayed marked heterogeneity indicating the existence of protein variants and isoenzymes. Principal pI values for the enzymes assayed were as follows: xanthine oxidase, 7.35--7.55; NADH2: iodonitrotetrazolium reductase, less than 4.5; 5'-nucleotidase, 7.15--7.4; alkaline phosphatase, 5.4--5.7; phosphodiesterase, 4.6--4.8; gamma-glutamyl transpeptidase, 4.4--4.55. 3. Fractions after electrofocusing were analyzed by 'fused rocket' immunoelectrophoresis and crossed immunoelectrophoresis after separation in polyacrylamide gels containing sodium dodecyl sulphate. Major antigens of the membrane include xanthine oxidase and glycoproteins of apparent molecular weights 67 000, 49 500 and 46 000. The latter two components sh...Continue Reading

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Citations

Sep 1, 1992·Journal of Neuroscience Research·M V GardinierJ M Matthieu
Apr 16, 1982·Molecular and Cellular Biochemistry·C H SullivanP J Madara
Feb 22, 2002·European Journal of Biochemistry·Kenji OshimaTsukasa Matsuda
Jun 6, 2002·European Journal of Biochemistry·Lone T PallesenJan T Rasmussen
May 24, 2013·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Bo Liu, David S Newburg
Aug 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Maria CavalettoAmedeo Conti
Jan 1, 1995·Journal of Dairy Science·H H Kim, R Jimenez-Flores
Sep 7, 2002·Journal of Dairy Science·C P WilcoxH E Swaisgood
Jul 24, 1998·Protein Expression and Purification·V L Spitsberg, R C Gorewit
Nov 5, 1999·Archives of Biochemistry and Biophysics·J L McManamanR M Wright
Nov 11, 1996·Biochimica Et Biophysica Acta·L BerglundJ T Rasmussen

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