Mar 1, 1975

Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients

Proceedings of the National Academy of Sciences of the United States of America
W M HolmesG W Hatfield

Abstract

The transfer ribonucleic acids of Escherichia coli bind to unsubstituted Sepharose in the presence of high concentrations of ammonium sulfate at pH 4.5. Transfer RNA species are eluted individually from the Sepharose by a gradient from high to low concentrations of ammonium sulfate; leucine tRNA is fractionated into five isoaccepting species. The order of elution of these isoaccepting species differs from that seen with reverse phase chromatography. By means of only these two procedures, one isoaccepting species of leucine tRNA can be purified to apparent homogeneity. Isoaccepting tRNA species for 9 amino acids have been resolved. This established the general utility of this chromatographic system for the separation and purification of specific isoaccepting transfer RNAs.

  • References8
  • Citations124

References

  • References8
  • Citations124

Mentioned in this Paper

Leucine tRNA
Alkalescens-Dispar Group
Amino Acids, I.V. solution additive
MT-TA gene
Triplet Codon-amino Acid Adaptor Activity
Transfer RNA
Chromatography, Agarose
Purification Aspects
Sepharose
Escherichia coli

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