PMID: 6538892Mar 1, 1984Paper

Separation of viable microfilariae free of blood cells on Percoll gradients

Journal of Helminthology
R ChandrashekarD Subrahmanyam

Abstract

A consistent and reproducible method is described for isolating pure populations of microfilariae of Litomosoides carinii, Brugia pahangi, B. malayi and Dipetalonema viteae, free of cells, from blood, by density gradient centrifugation on Percoll in 0.25 M sucrose. The recovery of the microfilariae was 85 to 97%.

References

Aug 1, 1978·Journal of Clinical Microbiology·D SubrahmanyamC K Rao
Jan 1, 1975·Transactions of the Royal Society of Tropical Medicine and Hygiene·T C JonesL C Pedrosa
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·H FeldmeierD Schuh

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Citations

Feb 1, 1992·Acta Tropica·G BardehleS Stirm
Sep 1, 1989·International Journal for Parasitology·U R RaoA C Vickery
Jul 1, 1989·Molecular and Biochemical Parasitology·K H Swamy
Jan 29, 2002·International Journal for Parasitology·Alexander W PfaffWolfgang H Hoffmann
Jun 17, 2009·Infection and Immunity·Karita HaapasaloT Sakari Jokiranta
Jun 1, 1992·Applied Biochemistry and Biotechnology·Y M GomesL Monjour
Jan 1, 1987·Parasitology Research·G BardehleH Zahner
Jan 1, 1990·Parasitology Research·U R RaoD Subrahmanyam
Jan 1, 1991·Parasitology Research·T KlonischS Stirm
Nov 7, 2009·Parasitology Research·Kathryn G GriffithsMichelle L Michalski
Aug 1, 1988·Immunological Investigations·P B ParabD Subrahmanyam
Jan 19, 2011·Molecular and Biochemical Parasitology·Ulrike WagnerHorst Zahner
Dec 10, 2015·Parasitology·Praveen Kumar TripathiRakesh Sehgal
Nov 1, 1985·Parasite Immunology·R ChandrashekarD Subrahmanyam
Sep 15, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S L HotiP K Das
Oct 1, 1987·Immunology and Cell Biology·U R RaoD Subrahmanyam
Apr 23, 2010·Lab Animal·Kathryn G GriffithsMichelle L Michalski
Oct 1, 1988·Immunology and Cell Biology·G R RajasekariahD Subrahmanyam
Feb 1, 1990·Immunology and Cell Biology·R ChandrashekarD Subrahmanyam
Jan 1, 1990·Parasitology Research·R ChandrashekarD Subrahmanyam
Jan 14, 2021·Cell Reports·Alexandra EhrensMarc Peter Hübner

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