Sepsis induces specific changes in histone modification patterns in human monocytes

PloS One
Sebastian WeitererMarkus A Weigand

Abstract

Sepsis is a global burden and the primary cause of death in intensive care units worldwide. The pathophysiological changes induced by the host's systemic inflammatory response to infection are not yet fully understood. During sepsis, the immune system is confronted with a variety of factors, which are integrated within the individual cells and result in changes of their basal state of responsiveness. Epigenetic mechanisms like histone modifications are known to participate in the control of immune reactions, but so far the situation during sepsis is unknown. In a pilot approach, we performed combined chromatin immunoprecipitation followed by high-throughput sequencing to assess the genome-wide distribution of the chromatin modifications histone 3 lysine 4 and 27 trimethylation and lysine 9 acetylation in monocytes isolated from healthy donors (n = 4) and patients with sepsis (n = 2). Despite different underlying causes for sepsis, a comparison over promoter regions shows a high correlation between the patients for all chromatin marks. These findings hold true also when comparing patients to healthy controls. Despite the global similarity, differential analysis reveals a set of distinct promoters with significant enrichment or d...Continue Reading

References

Feb 1, 1995·The Journal of Experimental Medicine·C H Chang, R A Flavell
Jan 21, 2006·Critical Care Medicine·Jean-Louis VincentUNKNOWN Sepsis Occurrence in Acutely Ill Patients Investigators
Jul 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hnin Thanda AungTimothy Ravasi
Feb 27, 2007·Cell·Tony Kouzarides
Feb 27, 2007·Intensive Care Medicine·Christoph EngelKonrad Reinhart
Nov 21, 2007·Molecular Medicine·Guillaume MonneretAlain Lepape
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
May 22, 2009·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Li ZhangYaping Wang
Sep 26, 2009·The EMBO Journal·Francesca De SantaGioacchino Natoli
Jun 30, 2010·PloS One·Kenneth Lyn-KewTheodore J Standiford
Jul 20, 2010·Bioinformatics·W J KentD Karolchik
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Nov 12, 2010·Nature·Edwige NicodemeAlexander Tarakhovsky
Dec 15, 2010·Intensive Care Medicine·Mark W HallJoseph A Carcillo
Jan 20, 2011·Genes & Development·Gioacchino NatoliIros Barozzi
Feb 12, 2011·Critical Care : the Official Journal of the Critical Care Forum·Lulong BoXiaoming Deng
Oct 11, 2011·Nature Reviews. Immunology·Chao Shi, Eric G Pamer
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xuefen ChenGioacchino Natoli
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Junhee SeokUNKNOWN Inflammation and Host Response to Injury, Large Scale Collaborative Research Program
Feb 23, 2013·The Lancet Infectious Diseases·Richard S HotchkissDidier Payen
Oct 8, 2013·Critical Care : the Official Journal of the Critical Care Forum·Sara CajanderJan Källman
Feb 25, 2014·Critical Care Medicine·Callie M ThompsonGrant E O'Keefe

❮ Previous
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Citations

Feb 6, 2016·Future Microbiology·Orhan Rasid, Jean-Marc Cavaillon
Jan 26, 2016·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Toshiaki IbaThachil Jecko
Apr 9, 2016·Critical Care : the Official Journal of the Critical Care Forum·C ArensF Uhle
Oct 7, 2016·Current Infectious Disease Reports·Manu Shankar-Hari, Gordon D Rubenfeld
Nov 10, 2017·Frontiers in Immunology·Fabiano Pinheiro da Silva, Marcel Cerqueira César Machado
Dec 24, 2018·Expert Review of Clinical Immunology·Niklas BruseMatthijs Kox
Aug 10, 2019·Anesthesia and Analgesia·Ettore CrimiClaudio Napoli
Sep 22, 2018·Shock·Jean-Marc Cavaillon, Evangelos J Giamarellos-Bourboulis
Oct 6, 2019·Life Science Alliance·William J CrislerLaurel L Lenz
May 13, 2020·Epigenomics·Jesús Beltrán-GarcíaJosé Luis García-Giménez
Nov 6, 2017·Archives of Gynecology and Obstetrics·C KouskoutiF Kainer
Nov 22, 2018·Frontiers in Immunology·Scott J DenstaedtTheodore J Standiford
Jun 19, 2017·Molecular Diagnosis & Therapy·Raymond J Langley, Hector R Wong
Feb 18, 2021·Expert Review of Clinical Immunology·Emily da Silva CórneoFelipe Dal-Pizzol
Mar 2, 2021·Expert Opinion on Therapeutic Targets·Elizabeth W TindalAlfred Ayala
Jul 30, 2021·Frontiers in Medicine·Renata Brito Falcão-HolandaReinaldo Salomão

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Datasets Mentioned

BETA
GSE62908

Methods Mentioned

BETA
acetylation
immunoprecipitation
ChIP-seq
density gradient centrifugation
flow cytometry
immunoprecipiation
PCR
Assay

Software Mentioned

DAVID
bigWigSummary
Genome browser
Genome Analyzer Pipeline Analysis
BOWTIE
R
DESeq
FastQC
BioConductor

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