SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells

PloS One
Chung-Hsin YehYing-Hung Lin

Abstract

Male infertility affects approximately 50% of all infertile couples. The male-related causes of intracytoplasmic sperm injection failure include the absence of sperm, immotile or immature sperm, and sperm with structural defects such as those caused by premature chromosomal condensation and DNA damage. Our previous studies based on a knockout mice model indicated that SEPT12 proteins are critical for the terminal morphological formation of sperm. SEPT12 mutations in men result in teratozospermia and oligozospermia. In addition, the spermatozoa exhibit morphological defects of the head and tail, premature chromosomal condensation, and nuclear damage. However, the molecular functions of SEPT12 during spermatogenesis remain unclear. To determine the molecular functions of SEPT12, we applied a yeast 2-hybrid system to identify SEPT12 interactors. Seven proteins that interact with SEPT12 were identified: SEPT family proteins (SEPT4 and SEPT6), nuclear or nuclear membrane proteins (protamine 2, sperm-associated antigen 4, and NDC1 transmembrane nucleoproine), and sperm-related structural proteins (pericentriolar material 1 and obscurin-like 1). Sperm-associated antigen 4 (SPAG4; also known as SUN4) belongs to the SUN family of protei...Continue Reading

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Citations

Sep 30, 2015·Developmental Biology·Alessandra CalviBrian Burke
Jun 18, 2016·Cells·Néjma BelaadiChristophe Guilluy
Nov 18, 2016·International Journal of Molecular Sciences·Tsung-Hsuan LaiYing-Hung Lin
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Feb 28, 2020·BioMed Research International·Hua NieWeibing Qin
Mar 15, 2019·International Journal of Molecular Sciences·Chung-Hsin YehYing-Hung Lin
Nov 25, 2020·Biomedicines·Yu-Hua LinYing-Hung Lin
Dec 3, 2020·International Journal of Molecular Sciences·Vera KmonickovaKaterina Komrskova
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May 1, 2021·Genes·Francesco ManfrevolaRosanna Chianese
May 20, 2021·Biopreservation and Biobanking·Samaneh FarajiRouhollah Fathi
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Jun 3, 2021·Nature Reviews. Urology·Changsheng DaiYu Sun
May 26, 2021·Current Biology : CB·Elias T Spiliotis, Konstantinos Nakos

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Methods Mentioned

BETA
2-hybrid
X-gal
PCR
coimmunoprecipitation
Co-IP
2-hybrids
immunoprecipitation

Software Mentioned

MetaMorph

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