Septins enforce morphogenetic events during sexual reproduction and contribute to virulence of Cryptococcus neoformans.

Molecular Microbiology
Lukasz Kozubowski, J Heitman

Abstract

Septins are conserved, cytoskeletal GTPases that contribute to cytokinesis, exocytosis, cell surface organization and vesicle fusion by mechanisms that are poorly understood. Roles of septins in morphogenesis and virulence of a human pathogen and basidiomycetous yeast Cryptococcus neoformans were investigated. In contrast to a well-established paradigm in S. cerevisiae, Cdc3 and Cdc12 septin homologues are dispensable for growth in C. neoformans yeast cells at 24 degrees C but are essential at 37 degrees C. In a bilateral cross between septin mutants, cells fuse but the resulting hyphae exhibit morphological abnormalities, including lack of properly fused specialized clamp cells and failure to produce spores. Interestingly, post-mating hyphae of the septin mutants have a defect in nuclear distribution. Thus, septins are essential for the development of spores, clamp cell fusion and also play a specific role in nuclear dynamics in hyphae. In the post-mating hyphae the septins localize to discrete sites in clamp connections, to the septa and the bases of the initial emerging spores. Strains lacking CDC3 or CDC12 exhibit significantly reduced virulence in a Galleria mellonella model of infection. Thus, C. neoformans septins are vi...Continue Reading

References

Apr 1, 1976·Journal of Bacteriology·K J Kwon-Chung, T J Popkin
Aug 1, 1985·The Journal of Clinical Investigation·D L GrangerD T Durack
Dec 1, 1993·Journal of Clinical Microbiology·J R PerfectC L Beiser
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·B L WickesJ C Edman
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·T H AdamsJ H Yu
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·L A Casselton, N S Olesnicky
Apr 26, 2000·Fungal Genetics and Biology : FG & B·R C DavidsonJ Heitman
Jul 7, 2001·Bioscience, Biotechnology, and Biochemistry·K OhsumiK Kitamoto
Dec 4, 2001·Current Opinion in Microbiology·A S GladfelterD J Lew
Jan 22, 2002·Medical Mycology·D C McFadden, A Casadevall
Jan 26, 2002·Molecular Biology of the Cell·Patrick J Westfall, Michelle Momany
Aug 16, 2002·Molecular Biology of the Cell·Amy J Warenda, James B Konopka
Nov 14, 2002·Annual Review of Genetics·Christina M Hull, Joseph Heitman
Dec 14, 2002·Developmental Cell·Makoto KinoshitaTimothy J Mitchison
Aug 13, 2003·Eukaryotic Cell·Indrani BoseTamara L Doering
Aug 23, 2003·Infection and Immunity·Kirsten NielsenJoseph Heitman
May 27, 2004·Journal of Basic Microbiology·Suzanna M BadalyanUrsula Kües
Sep 24, 2004·Molecular Biology of the Cell·Hanbing AnKathleen L Gould
Sep 29, 2004·FEMS Yeast Research·Guilhem Janbon
Oct 21, 2004·The Journal of Pathology·Peter A Hall, S E Hilary Russell
Feb 25, 2005·Molecular Biology of the Cell·Yong-Sun BahnJoseph Heitman
Mar 24, 2005·Journal of Cell Science·Amy S GladfelterDaniel J Lew
Jun 24, 2005·Infection and Immunity·Eleftherios MylonakisAndrew Diener
Jul 13, 2005·Trends in Cell Biology·Matthias Versele, Jeremy Thorner
Sep 2, 2005·British Journal of Cancer·S E H Russell, P A Hall
Sep 10, 2005·Eukaryotic Cell·Lois M DouglasJames B Konopka
Dec 24, 2005·Journal of Cell Science·Elias T Spiliotis, W James Nelson
Jan 24, 2006·Nature Reviews. Microbiology·Amy S Gladfelter
Jul 22, 2006·Microbiology and Immunology·Alberto González-NovoJavier Jiménez
Aug 11, 2006·Molecular Biology of the Cell·Hanspeter Helfer, Amy S Gladfelter
Aug 31, 2006·International Review of Cytology·Steven D Harris
Oct 28, 2006·Current Opinion in Microbiology·Rebecca Lindsey, Michelle Momany
Jul 3, 2007·BMC Evolutionary Biology·Fangfang PanMichelle Momany

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Citations

Nov 18, 2010·PloS One·Marcel NakahiraJörg Kobarg
Feb 24, 2016·Drug Discoveries & Therapeutics·Masaki IshiiKazuhisa Sekimizu
Jun 11, 2011·FEMS Microbiology Reviews·Lukasz Kozubowski, Joseph Heitman
Nov 21, 2015·PLoS Genetics·Nadia ChackoXiaorong Lin
Sep 10, 2016·PLoS Pathogens·Hee-Soo ParkMaria E Cardenas
Oct 26, 2013·Expert Review of Vaccines·Ashok K Chaturvedi, Floyd L Wormley
Jun 1, 2014·Current Opinion in Microbiology·Andrew A Bridges, Amy S Gladfelter
Jul 12, 2012·Mycobiology·Kyla Selvig, J Andrew Alspaugh
Jun 3, 2016·Journal of Cell Science·Sabrina ZanderMichael Feldbrügge
Sep 3, 2011·Traffic·Elias T Spiliotis, Amy S Gladfelter
Oct 4, 2017·Infection and Immunity·Deepa SrikantaTamara L Doering
Apr 21, 2017·Frontiers in Cell and Developmental Biology·Michelle Momany, Nicholas J Talbot
Oct 22, 2015·Annual Review of Microbiology·Anum KhanAmy S Gladfelter

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