PMID: 9188560Jul 1, 1997Paper

Sequence- and structure-specific determinants in the interaction between the RNA encapsidation signal and reverse transcriptase of avian hepatitis B viruses

Journal of Virology
J Beck, M Nassal

Abstract

Hepatitis B viruses (HBVs) replicate by reverse transcription of an RNA intermediate. Packaging of this RNA pregenome into nucleocapsids and replication initiation depend crucially on the interaction of the reverse transcriptase, P protein, with the cis-acting, 5' end-proximal encapsidation signal epsilon. The overall secondary structure is similar in all of the hepadnaviral epsilon signals, with a lower and an upper stem, separated by a bulge, and an apical loop. However, while epsilon is almost perfectly conserved in all mammalian viruses, the epsilon signals of duck HBV (DHBV) and heron HBV (D epsilon and H epsilon, respectively) differ substantially in their upper stem regions, both in primary sequence and in secondary structure; nonetheless, H epsilon interacts productively with DHBV P protein, as shown by its ability to stimulate priming, i.e., the covalent attachment of a deoxynucleoside monophosphate to the protein. In this study, we extensively mutated the variable and the conserved positions in the upper stem of D epsilon and correlated the functional activities of the variant RNAs in a priming assay with secondary structure and physical P protein binding. These data revealed a proper overall structure, with the bulge...Continue Reading

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Citations

Dec 19, 1998·Journal of Viral Hepatitis·A Kramvis, M C Kew
Dec 21, 2012·Journal of Virology·Scott A Jones, Jianming Hu
Mar 24, 1999·Journal of Biomedical Science·B B Oude Essink, B Berkhout
Apr 14, 2007·Nucleic Acids Research·Frederic C GirardSybren S Wijmenga
Oct 29, 2002·The Journal of Biological Chemistry·Shiow-Yi ChenYan-Hwa Wu Lee
Jul 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Joseph Che-Yen WangAdam Zlotnick
Aug 21, 2002·Journal of Virology·Kristin M Ostrow, Daniel D Loeb
Mar 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jürgen BeckMichael Nassal

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