PMID: 7547707May 1, 1995Paper

Sequence analysis and antigen binding characteristics of Ig SCID Ig+ mice

International Immunology
D Young, J F Kearney

Abstract

SCID mice as they age may develop a limited number of functional B and T cell clones. VH and VL gene sequences of hybridoma antibodies derived from the spleens of five adult SCID Ig+ mice revealed nine clonally unrelated, i.e. derived from different precursor lymphocytes, groups of Ig. Of these, four used the VH7183 family, one Q52, three J558 and one VH3609. VH7183 was seen in excess over J558 despite a 5-fold larger number of J558 family members in the mouse genome. Preferential use of D proximal VH genes along with reduced N region addition in the heavy chain VDJ joins of these antibodies suggest derivation from a population of lymphocytes with characteristics of the perinatal repertoire. All of the hybridomas produced kappa light chains of several different VL family groups. Sequences of 23 VH and 22 VL genes of hybridomas derived from one mouse (no. 45) showed that they were all clonally related, differing only in somatic mutations. Autoreactivity of these antibodies to cellular antigens [including (U1)RNP-specific A protein and SmB] was analyzed, and shown to correlate with the geneology and specific mutations within members of the clone. The geneological tree derived from sequence data permitted a study of the microevolu...Continue Reading

Citations

Dec 1, 1995·Current Opinion in Immunology·R J CornallJ G Cyster
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·C C Goodnow
Dec 18, 2002·The Journal of Experimental Medicine·Yijin LiMartin Weigert
Feb 6, 2004·The Journal of Experimental Medicine·Yijin LiMartin Weigert
Mar 6, 2003·Journal of Bacteriology·Christopher SteichenCharles L Turnbough
May 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa K SwieckiJohn F Kearney
Jun 6, 1998·Clinical Immunology and Immunopathology·X WuD C Young
Oct 1, 1996·Molecular and Cellular Biology·J S DanskaC J Guidos

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