PMID: 8467075Mar 1, 1993Paper

Sequence analysis of 22 kDa-like alpha-coixin genes and their comparison with homologous zein and kafirin genes reveals highly conserved protein structure and regulatory elements

Plant Molecular Biology
L M OttoboniP Arruda

Abstract

Several genomic and cDNA clones encoding the 22 kDa-like alpha-coixin, the alpha-prolamin of Coix seeds, were isolated and sequenced. Three contiguous 22 kDa-like alpha-coixin genes designated alpha-3A, alpha-3B and alpha-3C were found in the 15 kb alpha-3 genomic clone. The alpha-3A and alpha-3C genes presented in-frame stop codons at position +652. The two genes with truncated ORFs are flanking the alpha-3B gene, suggesting that the three alpha-coixin genes may have arisen by tandem duplication and that the stop codon was introduced before the duplication. Comparison of the deduced amino acid sequences of alpha-coixin clones with the published sequences of 22 kDa alpha-zein and 22 kDa-like alpha-kafirin revealed a highly conserved protein structure. The protein consists of an N-terminus, containing the signal peptide, followed by ten highly conserved tandem repeats of 15-20 amino acids flanked by polyglutamines, and a short C-terminus. The difference between the 22 kDa-like alpha-prolamins and the 19 kDa alpha-zein lies in the fact that the 19 kDa protein is exactly one repeat motif shorter than the 22 kDa proteins. Several putative regulatory sequences common to the zein and kafirin genes were identified within both the 5' a...Continue Reading

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Citations

Dec 1, 1996·Plant Molecular Biology·R T DeRoseT C Hall
Apr 2, 2008·Plant Molecular Biology·Pau MarzábalM Dolores Ludevid
Nov 12, 2010·Transgenic Research·Nicolau B CunhaElíbio L Rech
Nov 12, 2010·Transgenic Research·Nicolau B CunhaElíbio L Rech
Nov 10, 1998·The Plant Journal : for Cell and Molecular Biology·P MarzábalM Torrent
Dec 31, 2004·Nucleic Acids Research·Rentao SongJoachim Messing
Oct 7, 2010·Genome Génome / Conseil National De Recherches Canada·Xiangzong MengRentao Song
Oct 28, 1994·Molecular & General Genetics : MGG·F A de FreitasA Leite
Feb 13, 2020·Plants·Veronica RutaPaola Vittorioso
Jan 1, 1996·Annual Review of Phytopathology·A H Purcell, D L Hopkins

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