PMID: 6171821Nov 1, 1981Paper

Sequence analysis of a cDNA coding for a pancreatic precursor to somatostatin

Proceedings of the National Academy of Sciences of the United States of America
W L TaylorJ E Dixon

Abstract

A synthetic oligonucleotide having the sequence d(T-T-C-C-A-G-A-A-G-A-A) deduced from the amino acid sequence Phe-Phe-Trp-Lys of somatostatin-14 was used to prime the synthesis of a cDNA from channel catfish (Ictalurus punctatus) pancreatic poly(A)-RNA. The major product of this reaction was a cDNA fragment of 565 nucleotides. Chemical sequence analysis of the cDNA fragment revealed that it was complementary to a mRNA coding for somatostatin. The 565-nucleotide cDNA hybridizes strongly with a poly(A)-RNA estimated to be 1000 nucleotides in length. An amino acid sequence of the somatostatin precursor was predicted from the nucleotide sequence. Oyama et al. [Oyama, H., Bradshaw, R. A., Bates, O. J. & Permutt, A. (1980) J. Biol. Chem. 255, 2251-2254] have reported the isolation of a somatostatin from the catfish that is 22 residues in length (somatostatin-22). This peptide differs from somatostatin-14 in amino acid sequence. The cDNA sequence obtained by this laboratory codes for somatostatin-14 and predicts another somatostatin gene product from this species. Thus it would appear that there are at least two somatostatin gene products.

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Citations

Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·X W LinR E Peter
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L P ShenW J Rutter
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M MagazinJ E Dixon
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I Sures, M Crippa
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R J DeschenesJ E Dixon
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J Spiess, B D Noe
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C L Funckes-ShippyA D Levine
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·X C LuoK H Kim
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·X LinR E Peter

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