PMID: 9544777Apr 17, 1998Paper

Sequence analysis of a compound coding-region microsatellite in Candida albicans resolves homoplasies and provides a high-resolution tool for genotyping

FEMS Immunology and Medical Microbiology
D MetzgarC Wills

Abstract

Sequence diversity at a coding-region microsatellite locus of two diploid Candida species was surveyed. Twenty-one alleles from fourteen strains of Candida albicans and three alleles from two strains of the closely related Candida dubliniensis were sequenced. Results show independent length variation in two contiguous hexanucleotide repeats, one non-contiguous hexanucleotide repeat, and two non-contiguous trinucleotide repeats within a 120 bp coding region. A neighboring, non-repetitive 120 bp region showed no variation. The information density of sequence polymorphisms in this region provides a powerful tool for genotyping microorganisms in epidemiological studies, yielding detailed resolution of closely related strains, and clearly distinguishing the two species studied here. The individual length-variable repeat regions are very short (2-8 repeats), demonstrating that even very short microsatellites can show high levels of length variability when surrounded by similarly repetitive DNA. Extensive homoplasy was discovered among the C. albicans alleles, with the majority of overall length categories consisting of alleles with more than one sequence. Our results show that microsatellite length alone should not be used to assume ...Continue Reading

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Citations

Feb 15, 2000·Research in Microbiology·G F RichardB Dujon
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ruth E FundygaJonathan Arnold
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Apr 9, 2004·Journal of Clinical Microbiology·Brent A Lasker, Yuping Ran
Jan 15, 2013·Journal of Applied Microbiology·F SaghrouniM Ben Said
Jul 12, 2013·BioMed Research International·Claudia Spampinato, Darío Leonardi
Jun 29, 1999·Current Opinion in Microbiology·A van Belkum
Jul 14, 2010·Journal of Microbiological Methods·Marcelo Fabiano Gomes BoriolloAline Aparecida Pizzirani-Kleiner
Mar 4, 2015·BioMed Research International·Joana Carvalho-PereiraPaula Sampaio
Dec 18, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bin GaoShunyi Zhu
Sep 18, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shunyi Zhu, Bin Gao
Apr 17, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiajia WuShunyi Zhu
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May 24, 2001·Molecular Biology and Evolution·M CulverS J O'Brien
Dec 22, 2009·Future Microbiology·Frank C Odds
Oct 7, 2004·Fungal Genetics and Biology : FG & B·S LimD A Carter

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