PMID: 9178269May 1, 1997Paper

Sequence analysis of a gene family encoding Taenia ovis vaccine antigens expressed during embryogenesis of eggs

Molecular and Biochemical Parasitology
J G WaterkeynMarshall W Lightowlers

Abstract

The 45W vaccine antigen of the cestode parasite Taenia ovis is a member of a small gene family estimated to comprise six members. All six genes were cloned and characterised. Nucleotide sequence analysis of the different family members identified a set of closely related genes exhibiting between 75.2 and 98.6% DNA sequence homology. Intron/exon structure for the various genes constituting the family was found to be conserved. Open reading frame analysis and pairwise alignments of predicted polypeptides from the various members of the family revealed that the encoded proteins share between 51.8 and 96.5% amino acid identity with the host-protective 45W antigen. Polymerase chain reaction (PCR) primers were designed which allowed amplification of individual family members in a transcript-specific manner. These were used to investigate gene expression in different developmental stages of the parasite by reverse-transcription polymerase chain reaction (RT-PCR). All members of the gene family were expressed during the T. ovis life-cycle. 45W gene family transcription correlates with embryogenesis of eggs and expression is increased in activated eggs (oncospheres). One family member, ToW3S, was expressed in the early larvae (cysticerc...Continue Reading

Citations

Sep 19, 2003·International Journal for Parasitology·M W LightowlersA J Read
Sep 10, 1999·International Journal for Parasitology·M W Lightowlers
Apr 4, 2003·Molecular and Biochemical Parasitology·Charles Gauci, Marshall W Lightowlers
Apr 27, 2000·Parasitology Today·M W LightowlersR Rolfe
Nov 15, 2001·Veterinary Parasitology·M W Lightowlers, C G Gauci
Jul 25, 2003·Veterinary Parasitology·M W LightowlersC Sato
Aug 24, 1999·Veterinary Parasitology·R B Gasser
Aug 24, 2004·Infection and Immunity·Ana FlisserMarshall W Lightowlers
Jan 5, 2011·PLoS Neglected Tropical Diseases·Jonas LundströmJohan Lindh
Sep 14, 2007·Molecular and Biochemical Parasitology·Holger MaytaUNKNOWN Cysticercosis Working Group in Peru
Jun 3, 2004·Experimental Parasitology·Conan ChowMarshall W Lightowlers
May 1, 1997·Experimental Parasitology·J G WaterkeynM W Lightowlers
Mar 21, 1998·Experimental Parasitology·J G WaterkeynM W Lightowlers
Jan 10, 2008·Trends in Parasitology·Karen L HaagFrancisco J Ayala

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