Sequence analysis of alginate-derived oligosaccharides by negative-ion electrospray tandem mass spectrometry

Journal of the American Society for Mass Spectrometry
Zhenqing ZhangW Chai

Abstract

Negative-ion electrospray tandem mass spectrometry (ES-MS/MS) with collision-induced dissociation (CID) is attempted for sequence determination of alginate oligosaccharides, derived from polyanionic alginic acid, polymannuronate, and polyguluronate by partial depolymerization using either alginate lyase or mild acid hydrolysis. Sixteen homo- and hetero-oligomeric fragments were obtained after fractionation by gel-filtration and strong anion exchange high performance liquid chromatography. The product-ion spectra of these alginate oligosaccharides were dominated by intense B-, C-, Y-, and Z-type ions together with (0,2)A- and (2,5)A-ions of lower intensities. Internal mannuronate residues (M) produce weak but specific decarboxylated Z(int)-ions (Z(int) - 44 Da; int: denotes internal), which can be used for distinction of M and a guluronate residue (G) at an internal position. A reducing terminal M or G, although neither gives rise to a specific ion, can be identified by differences in the intensity ratio of fragment ions of the reducing terminal residue [(2,5)A(red)]/[(0,4)A(red)] (red: denotes reducing terminal).

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Citations

Oct 22, 2009·Applied Microbiology and Biotechnology·Ryoji MatsushimaMasataka Tsuda
Dec 7, 2007·Journal of the American Society for Mass Spectrometry·Zhenqing ZhangRobert J Linhardt
Dec 20, 2012·Journal of Agricultural and Food Chemistry·Melliana C JonathanHarry Gruppen
Nov 9, 2006·Bioscience, Biotechnology, and Biochemistry·Takamitsu ChakiTakashi Baba
Feb 25, 2014·International Journal of Biological Macromolecules·Hamed DaemiMohammad Barmar
Feb 24, 2009·Carbohydrate Research·Stanislav D AnastyukTatyana N Zvyagintseva
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Jun LiuZhengqiang Jiang
Jun 14, 2021·Carbohydrate Polymers·Ushasree Mrudulakumari VasudevanEun Yeol Lee
Jul 14, 2021·Critical Reviews in Food Science and Nutrition·Chunhua ZhangChunpeng Wan
Aug 9, 2021·Carbohydrate Polymers·Mingpeng WangZhaojie Zhang

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