PMID: 7545934May 15, 1994Paper

Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector

FEMS Microbiology Letters
E KinnerH Schmieger

Abstract

The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram-positive bacteria that have been studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus.

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