PMID: 8585089Sep 1, 1995Paper

Sequence analysis of fibrolase, a fibrinolytic metalloproteinase from Agkistrodon contortrix contortrix

Toxicon : Official Journal of the International Society on Toxinology
M C Manning

Abstract

Fibrolase is a small (203 amino acids), nonhemorrhagic, fibrinolytic enzyme from the venom of Agkistrodon contortrix contortrix (southern copperhead). While the chemical and physical properties of the protein have been extensively studied, its overall globular structure is unknown. By comparison with homologous metalloproteinases and snake toxins, the catalytic zinc binding site of fibrolase has been identified, as well as a potential binding site for calcium, which has not been recognized before. The positions of the major secondary structural features are predicted, and found to be similar to other structurally characterized metalloproteinases, while the positions of the three intramolecular disulfide bonds are also postulated. Finally, fibrolase is reported to be nonhemorrhagic and earlier work on hemorrhagic enzymes from snake venoms identified six amino acids which might be responsible for hemorrhagic activity. It is shown here that most of these residues occur in fibrolase, and yet it is nonhemorrhagic in its activity. Altogether, this work demonstrates the utility of sequence analysis methods in the characterization of the structure of venom-derived proteins.

References

May 11, 1992·Protein Science : a Publication of the Protein Society·A RandolphF R Masiarz
Jul 25, 1990·Nucleic Acids Research·M P Neeper, M A Jacobson
Dec 15, 1986·The Biochemical Journal·S E WhithamA J Docherty
Jun 1, 1994·Protein Science : a Publication of the Protein Society·E E HodgkinR J Gilbert
Jul 15, 1993·Biochemical and Biophysical Research Communications·D L GerloffS A Benner

❮ Previous
Next ❯

Citations

May 11, 2005·Toxicon : Official Journal of the International Society on Toxinology·S Swenson, F S Markland
May 23, 2001·Protein Science : a Publication of the Protein Society·G JonesZ Zhang
May 27, 2005·Acta Pharmacologica Sinica·Yi-wen WangGuang-mei Yan
May 19, 2006·Pathophysiology of Haemostasis and Thrombosis·Steven R Deitcher, Christopher F Toombs
Oct 20, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Dayane L Naves de SouzaVeridiana M Rodrigues
Jan 17, 2007·Expert Opinion on Biological Therapy·Steven R DeitcherChristopher F Toombs
Sep 15, 2012·Toxicon : Official Journal of the International Society on Toxinology·Nadia C G de MoraisFábio de Oliveira
Jan 26, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·O H P Ramos, H S Selistre-de-Araujo
Sep 29, 2004·Toxicon : Official Journal of the International Society on Toxinology·O H P Ramos, H S Selistre-de-Araujo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.