Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var israelensis ATCC35646.

Genetics Research International
Bouziane MoumenAlexei Sorokin

Abstract

Diarrheic food poisoning by bacteria of the Bacillus cereus group is mostly due to several toxins encoded in the genomes. One of them, cytotoxin K, was recently identified as responsible for severe necrotic syndromes. Cytotoxin K is similar to a class of proteins encoded by genes usually annotated as haemolysin II (hlyII) in the majority of genomes of the B. cereus group. The partially sequenced genome of Bacillus thuringiensis var israelensis ATCC35646 contains several potentially induced prophages, one of them integrated into the hlyII gene. We determined the complete sequence and established the genomic organization of this prophage-designated phIS3501. During induction of excision of this prophage with mitomycin C, intact hlyII gene is formed, thus providing to cells a genetic ability to synthesize the active toxin. Therefore, this prophage, upon its excision, can be implicated in the regulation of synthesis of the active toxin and thus in the virulence of bacterial host. A generality of selection for such systems in bacterial pathogens is indicated by the similarity of this genetic arrangement to that of Staphylococcus aureus  β-haemolysin.

References

Dec 25, 1995·Nucleic Acids Research·J K BonfieldR Staden
Mar 21, 1998·Nucleic Acids Research·A V Lukashin, M Borodovsky
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·E SelkovM Fonstein
Apr 1, 2000·Microbes and Infection·A KotirantaM Haapasalo
Nov 9, 2000·Molecular Microbiology·T LundP E Granum
Sep 7, 2001·Annual Review of Microbiology·M Mock, A Fouet
Aug 23, 2002·Nature·Raymond SchuchVincent A Fischetti
Nov 12, 2002·Journal of Bacteriology·Claire M FraserSteven L Salzberg
Nov 12, 2002·Journal of Bacteriology·Elbert Branscomb, Paul Predki
Apr 16, 2003·Molecular Microbiology·Jeralyn D Haraldsen, Abraham L Sonenshein
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Carlos CanchayaHarald Brüssow
Mar 3, 2004·Microbiology·Benjamin CandelonAlexei Sorokin
May 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alex R HoffmasterClaire M Fraser
Aug 4, 2004·Microbiology·Annette FagerlundPer E Granum
Nov 13, 2004·Molecular Nutrition & Food Research·Monika Ehling-SchulzSiegfried Scherer
Apr 6, 2005·FEMS Microbiology Reviews·David A RaskoJacques Ravel
Aug 16, 2005·FEMS Microbiology Letters·Iain AndersonNatalia Ivanova
Sep 17, 2005·Journal of Bacteriology·Sophie DavisonAgnès Fouet
Oct 18, 2005·Journal of Molecular Biology·Leonid MinakhinKonstantin Severinov
Oct 26, 2005·Nucleic Acids Research·Shea N GardnerTom R Slezak
Dec 27, 2005·Antimicrobial Agents and Chemotherapy·Christiane GoerkeChristiane Wolz
Dec 29, 2005·Protein Expression and Purification·Zhanna I AndreevaAlexander S Solonin
Apr 20, 2006·Journal of Bacteriology·Derrick E FoutsSteven R Gill
Aug 16, 2006·Journal of Applied Microbiology·N J TourasseA-B Kolstø
Nov 3, 2006·Molecular Microbiology·Taeok BaeOlaf Schneewind
Dec 13, 2006·Nature Biotechnology·Vincent A FischettiRaymond Schuch
Mar 6, 2007·Journal of Bacteriology·Jean F ChallacombeThomas S Brettin
Apr 17, 2007·Chemico-biological Interactions·Alla LapidusAlexei Sorokin
May 23, 2007·BMC Microbiology·Annette FagerlundPer Einar Granum

❮ Previous
Next ❯

Citations

Nov 5, 2016·Research in Microbiology·Alexandre BolotinAlexei Sorokin
Nov 29, 2013·The Journal of General Virology·Yihui YuanYan Wu

❮ Previous
Next ❯

Datasets Mentioned

BETA
CM000759.1
JQ062992

Methods Mentioned

BETA
PCR

Software Mentioned

Xbap
Sequin
ERGOlight
GeneMark
Gap4
Pinned Regions
BLAST

Related Concepts

Related Feeds

Bacillus Cereus Infection

Bacillus Cereus is a gram-positive bacteria that is the cause of some foodborne illnesses and leads to diarrhea and vomiting. Discover the latest research on Bacillus Cereus Infection here.