Sequence analysis of the 5' third of glycoprotein C gene of South American bovine herpesviruses 1 and 5

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Carolina Kist TraeselE F Flores

Abstract

Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) share high genetic and antigenic similarities, but exhibit marked differences in tissue tropism and neurovirulence. The amino-terminal region of glycoprotein C (gC), which is markedly different in each of the viruses, is involved in virus binding to cellular receptors and in interactions with the immune system. This study investigated the genetic and antigenic differences of the 5' region of the gC (5' gC) gene (amino-terminal) of South American BoHV-1 (n=19) and BoHV-5 (n=25) isolates. Sequence alignments of 374 nucleotides (104 amino acids) revealed mean similarity levels of 97.3 and 94.2% among BoHV-1 gC (gC1), respectively, 96.8 and 95.6% among BoHV-5 gC (gC5), and 62 and 53.3% between gC1 and gC5. Differences included the absence of 40 amino acid residues (27 encompassing predicted linear epitopes) scattered throughout 5' gC1 compared to 5' gC5. Virus neutralizing assays testing BoHV-1 and BoHV-5 antisera against each isolate revealed a high degree of cross-neutralization between the viruses, yet some isolates were neutralized at very low titers by heterologous sera, and a few BoHV-5 isolates reacted weakly with either sera. The virus neutralization differences observed within...Continue Reading

References

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Feb 1, 2014·Virus Genes·Carolina Kist TraeselEduardo Furtado Flores

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Datasets Mentioned

BETA
AJ004801
AY261359

Methods Mentioned

BETA
glycosylation
PCR
protein folding

Software Mentioned

BioEdit Sequence Alignment Editor
BLAST
Staden Package
Sequence Manipulation Suite
BioEdit
MEGA
ClustalW

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