Sequence Analysis of the cAMP-Dependent Protein Kinase Regulatory Subunit-Like Protein From Trypanosoma brucei

Acta Parasitologica
Nelson A Araujo, J Bubis

Abstract

Study the N-terminal, C-terminal, and linker regions of the TbPKAr using homology modeling. The amino acid sequences of the N-terminal, C-terminal, and linker regions of the TbPKAr were individually examined by means of BLAST analysis and in silico secondary structure predictions with several programs. The TbPKAr C-terminal region, showed a well-folded α/β structure, which consists of two concurrent flattened β-barrel-shaped domains that are separated by an elongated central α-helix similar to its mammalian counterpart, the TbPKAr linker region contains a PKA phosphorylation site and was predicted to be rather disordered. Our analysis also indicated that the TbPKAr N-terminal region lacks a docking/dimerization domain but is enriched in motifs known as leucine-rich repeats (LRR). The replacement of the docking/dimerization domain by different structural motifs suggests the inability of TbPKAr to form homodimers; however, the function of the TbPKAr N-terminal LRR-containing domain in Kinetoplastidae parasites is still unknown.

References

Aug 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S S TaylorJ Sowadski
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Mar 22, 1982·FEBS Letters·O Zetterqvist, U Ragnarsson
Jan 1, 1996·Methods in Enzymology·J GarnierB Robson
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Mar 13, 1998·Molecular and Biochemical Parasitology·B ReunerM Boshart
May 23, 1998·Journal of Molecular Biology·A V Kajava
Dec 6, 2001·European Journal of Biochemistry·T ShalabyT Seebeck
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Jennifer J Carlisle Michel, John D Scott
Jan 30, 2004·Proteins·Purevjav EnkhbayarNorio Matsushima
Jun 25, 2004·Nucleic Acids Research·Burkhard RostJinfeng Liu
Jul 21, 2004·Computational Biology and Chemistry·B KnudsenJ Gorodkin
Mar 31, 2005·Bioinformatics·Taner Z SenAndrzej Kloczkowski
Jul 7, 2007·Cellular Signalling·Mandy DiskarFriedrich W Herberg
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
May 9, 2008·Nucleic Acids Research·Christian ColeGeoffrey J Barton
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
Mar 2, 2010·The Journal of Biological Chemistry·Stephen C ToveyColin W Taylor
Sep 21, 2012·Nature Reviews. Molecular Cell Biology·Susan S TaylorAlexandr P Kornev
Oct 30, 2013·Protein and Peptide Letters·Hiroki MiyashitaNorio Matsushima
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium
Nov 14, 2014·Bioinformatics·David T Jones, Domenico Cozzetto
Dec 31, 2014·Nature Methods·Jianyi YangYang Zhang

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Methods Mentioned

BETA
gel filtration
X-ray

Software Mentioned

Clustal W / X
TASSER
DISOPRED3
JPred3
GOR4
BLAST
PredictProtein
Phyre2
GOR

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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.