Sequence analysis of the Chlamydomonas reinhardtii flagellar alpha dynein gene

Cell Motility and the Cytoskeleton
David R Mitchell, K S Brown

Abstract

Flagellar outer row dynein ATPases have been used extensively as model systems for studies of microtubule-based motility. Previously full-length sequences were only available for two of the three catalytic heavy-chain subunits (DHCs) of this enzyme. We have completed the sequence of an 18-kb genomic region encoding the Chlamydomonas reinhardtii flagellar outer row dynein alpha heavy chain. Unlike the beta- and gamma-subunits, DHC alpha is not required for assembly of other outer row dynein proteins, except for a tightly associated light chain, and thus occupies a unique position within this enzyme complex. The predicted 4,499 residue protein retains sequence homology to other dynein heavy chains throughout its central and C-terminal regions but lacks homology to any other dyneins in the first 1,000 amino acids, which may account for its unusual assembly properties. This N-terminal domain of DHC alpha contains a repetitive sequence rich in alanines, prolines, and glutamic acids. Within the more homologous C-terminal region, which includes the catalytic domain, three short sequences unique to DHC alpha may account for its specific catalytic properties and in vivo phosphorylation pattern.

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Citations

Sep 3, 1998·Molecular Biology of the Cell·M E Fowkes, D R Mitchell
Mar 9, 1999·Molecular Biology of the Cell·M E PorterW Dentler
Apr 10, 2003·Eukaryotic Cell·Pushpa KathirCarolyn D Silflow
Dec 23, 2006·BMC Genomics·Ghazaleh NematollahiArmin Hallmann
Nov 24, 1999·The Journal of Eukaryotic Microbiology·W XuD G Pennock
Jul 12, 2002·Cell Motility and the Cytoskeleton·Alistair HarrisonStephen M King
Jul 17, 2014·Biophysical Journal·Shohei FujitaMasahide Kikkawa
Apr 8, 2004·The Journal of Eukaryotic Microbiology·David J Asai, David E Wilkes
Aug 3, 2017·Cold Spring Harbor Perspectives in Biology·Rasagnya ViswanadhaMary E Porter
Apr 29, 1998·Cell Motility and the Cytoskeleton·I Milisav

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