PMID: 3750843Oct 15, 1986Paper

Sequence analysis of the E2 gene of a hyperglycosylated, host restricted mutant of Sindbis virus and estimation of mutation rate from frequency of revertants

Virology
R K Durbin, V Stollar

Abstract

SVap15/21, a strain of Sindbis virus (SV) derived from our standard laboratory strain of SV (SVstd) after repeated passage on Aedes albopictus cells, grows normally in mosquito cells but is host restricted (hr) in vertebrate cells. It is also temperature sensitive (ts) and produces pinpoint plaques on vertebrate cells (sp). E2 glycoprotein of SVstd differs from that of the more widely used SVHR (from which SVstd was derived) by an additional (i.e., third) N-linked glycan. The E2 of SVap15/21, in turn, differs from that of SVstd by the addition of a fourth glycan. We have determined the nucleotide sequence of the E2 genes of SVap15/21 and of SVstd, as well as that of our isolate of SVHR. The nucleotide sequence of the SVstd E2 gene predicted the occurrence of an additional N-linked glycan attachment site, not present in the SVHR E2, at Asn232 (Asp in SVHR). The sequence of the SVap15/21 E2 gene demonstrated three mutations relative to the SVstd gene, including one that predicted the occurrence of another potential N-linked glycan attachment site at Asn275. Sequence analysis of 15 revertants of SVap15/21 which are no longer host-restricted revealed that all had lost the glycosylation site at Asn275, confirming the connection betw...Continue Reading

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Citations

Dec 5, 1988·Journal of Molecular Biology·A VillaverdeE Domingo
Sep 18, 2001·Journal of Virology·I PultJ Summers
Feb 27, 2014·Human Gene Therapy Methods·Gray KueberuwaLeonard W Seymour
Apr 1, 1993·In Vitro Cellular & Developmental Biology. Animal·V Stollar
Nov 30, 2004·Virus Research·Henrik GaroffR Holland Cheng
Aug 1, 1993·Journal of Virology·P C TuckerD E Griffin
Dec 1, 1990·Journal of Virology·J C de la Torre, J J Holland
Sep 1, 1988·Journal of Virology·J M LeiderF I Smith
Sep 1, 1994·Microbiological Reviews·J H Strauss, E G Strauss

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