Sequence analysis of the 'Goodpasture antigen' of mammals

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
J J RyanA Neil Turner

Abstract

Autoimmunity to the NC1 domain of the alpha3 chain of type IV collagen (alpha3(IV)NC1), the Goodpasture antigen, is the cause of spontaneous human antiglomerular basement membrane (anti-GBM) disease, and of anti-GBM nephritis in several animal models. We have derived amino acid sequences from alpha3(IV)NC1 for a number of mammalian species (monkey, sheep, pig, dog, rabbit, and rat) by RT-PCR and cDNA cloning. The GBM of some species was studied comparatively for binding to Goodpasture autoantibodies. From this work and other data the sequences of nine mammalian species can be aligned. Regions and residues that may be functionally important are identified. Alpha3(IV)NC1 sequences were found to be less closely conserved across species than alpha1 and alpha2(IV)NC1, 91 to 99% in comparison to a minimum of 97% for alpha1, but these differences were unevenly distributed along the molecule. There was a particularly striking homology between rodent and human sequences in the carboxyl-terminal region. Binding of Goodpasture autoantibodies to rat alpha3(IV)NC1 was poor in comparison with other species. Comparison of sequences and binding casts doubt on the importance of the carboxyl-terminal region for antibody binding, a region identif...Continue Reading

Citations

Aug 18, 2005·Kidney International·Julie RobertsonYa-Huan Lou
Jun 7, 2015·Journal of Translational Medicine·Inge M Worni-SchudelMary H Foster
Jul 29, 2005·Journal of the American Society of Nephrology : JASN·Warren Kline BoltonJay W Fox
Jul 30, 2005·Arthritis and Rheumatism·Iftikhar A ChowdhryBetty Diamond
Aug 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wentian LuoDorin-Bogdan Borza
Mar 16, 2017·Journal of Cellular and Molecular Medicine·Shui-Yi HuMing-Hui Zhao
Feb 7, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J J RyanC D Pusey
Mar 30, 2005·The Journal of Nutrition·M Pilar MarínJoaquín Timoneda
May 29, 2002·The Journal of Laboratory and Clinical Medicine·An-Ming LuoW Kline Bolton

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