PMID: 9524216Apr 3, 1998Paper

Sequence analysis of the Porphyromonas gingivalis dipeptidyl peptidase IV gene

Biochimica Et Biophysica Acta
M KiyamaY Abiko

Abstract

We previously constructed a Porphyromonas gingivalis genomic library and isolated the 2.9 kb EcoRV fragment which specified glycylprolyl dipeptidyl aminopeptidase (GPase). Nucleotide sequencing of this fragment identified the single 2169 bp open reading frame which coded for a 723 amino acid protein. The amino acid sequencing of the NH2-terminal domain of the native and recombinant mature enzymes suggested that the protease possessed a 16 amino acid residue signal peptide. The calculated mass of the precursor and mature proteases were 82,018 and 80,235 daltons, respectively. The homology search of this enzyme in registered protein sequences revealed that this enzyme was homologous to dipeptidyl peptidase (DPP) IV from the Flavobacterium meningosepticum and that from eukaryotic cells, including the human, mouse, and rat. Three amino acid residues, Ser-593, Asp-668, and His-700, were identified as a putative catalytic triad, a common feature of eukaryotic serine proteases. In addition, this enzyme showed a broad proteolytic spectrum toward synthetic substrates capable of splitting not only Gly-Pro-derivative but also Ala-Pro, Lys-Pro, and Phe-Pro-derivatives. Therefore, we conclude that this enzyme belongs to DPP IV rather than G...Continue Reading

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Citations

Mar 4, 2000·Journal of Periodontal Research·M A CurtisJ Aduse-Opoku
Sep 2, 2003·Journal of Bacteriology·Karen E NelsonClaire M Fraser
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A CurtisM Rangarajan
Jul 11, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuko OgawaRyohei Yanoshita
Jun 4, 2008·Biological & Pharmaceutical Bulletin·Yuko OgawaRyohei Yanoshita
Nov 30, 2000·The Journal of Biological Chemistry·A BanbulaJ Potempa
Nov 13, 2009·Journal of Periodontology·Piyamas AemaimananSuwimol Taweechaisupapong

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