PMID: 9008333Dec 1, 1996Paper

Sequence analysis of the putative viral enhancer in tissues from 33 cats with various feline leukemia virus-related diseases

Veterinary Microbiology
M L JacksonV Misra

Abstract

Diseases resulting from infection by feline leukemia virus (FeLV) and several other retroviruses relate in part, to non-coding regulatory sequences within the viral long terminal repeat (LTR). Both enhancer repeats and mutations within the LTR have been implicated in FeLV related disease. In order to investigate the relationship between nucleotide sequence of the FeLV LTR and disease, tissues from 33 cats with different types of degenerative and proliferative FeLV-related disease were studied. An FeLV LTR region containing the putative transcriptional enhancer unit was amplified by polymerase chain reaction (PCR) from FeLV-infected tissues. Phylogenetic analysis of FeLV 3'unique (U3) sequences revealed only one meaningful grouping which contained 4 of the 5 antigen-negative lymphosarcomas (LSAs). No sequence duplications were found in any of the 33 FeLV U3 regions. Point mutations relative to the corresponding region of FeLV-A/Glasgow, were identified at 102 positions; 68 of these were accounted for by mutations at 5 locations. Only 1 point mutation was found within the leukemia virus b-simian virus 40-like core (LVb-CORE) site. However, the nuclear factor 1 (NF1) site contained 11 mutations, and the FeLV-specific (FLV-1) site ...Continue Reading

References

Jan 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D M Haines, B J Chelack
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N RiedelJ I Mullins
Jul 11, 1988·Nucleic Acids Research·B Hallberg, T Grundström
Aug 1, 1973·The Journal of General Virology·O JarrettD Hay
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J W CaseyN Davidson
Jan 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M L JacksonV Misra

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Citations

Feb 22, 2008·Veterinary Immunology and Immunopathology·Laura S Levy
Mar 9, 2004·Journal of Comparative Pathology·M J DayN J Bacon
Dec 7, 2010·Journal of Feline Medicine and Surgery·Bianca StützerKatrin Hartmann
Sep 28, 2004·The Journal of General Virology·Chandtip ChandhasinLaura S Levy

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