PMID: 11928991Apr 4, 2002Paper

Sequence analysis of the Washington/1964 strain of human parainfluenza virus type 1 (HPIV1) and recovery and characterization of wild-type recombinant HPIV1 produced by reverse genetics

Virus Genes
Jason T NewmanMario H Skiadopoulos

Abstract

A complete consensus sequence was determined for the genomic RNA of human parainfluenza virus type 1 (HPIV1) strain Washington/20993/1964 (HPIV1 WASH/64), a clinical isolate that previously was shown to be virulent in adults. The sequence exhibited a high degree of relatedness to both Sendai virus, a PIV1 virus recovered from mice, and human PIV3 (HPIV3) with regard to cis-acting regulatory regions and protein-coding sequences. This consensus sequence was used to generate a full-length antigenomic cDNA and to recover a recombinant wild-type HPIV1 (rHPIV1). Interestingly, the rHPIV1 could be rescued from full-length antigenomic rHPIV1 cDNA using HPIV3 support plasmids, HPIV1 support plasmids, or a mixture thereof. The replication of rHPIV1 in vitro and in the respiratory tract of hamsters was similar to that of its biologically derived parent virus. The similar biological properties of rHPIV1 and HPIV1 WASH/64 in vitro and in vivo, together with the previous demonstration of the virulence of this specific isolate in humans, authenticates the rHPIV1 sequence as that of a wild-type virus. This rHPIV1 can now be used to study the biological properties of HPIV1 and as a substrate to introduce attenuating mutations for the generation...Continue Reading

Citations

Dec 23, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Anna P Durbin, Ruth A Karron
Aug 24, 2011·Expert Review of Respiratory Medicine·Alexander C SchmidtPeter L Collins
Jun 16, 2016·Journal of Medical Microbiology·Tanja Košutić-GulijaDubravko Forčić
Aug 7, 2013·The Journal of General Virology·Susanna K P LauKwok-Yung Yuen
Apr 14, 2017·MSphere·Shannon M BeatyBenhur Lee
Apr 10, 2014·The Journal of Clinical Investigation·Rachael M LiesmanRaymond J Pickles
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
Nov 29, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Giuseppe GernaM Grazia Revello
Feb 11, 2011·Virology Journal·Hui-Ting YangCheng-Qiang He

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