PMID: 9188616Jul 1, 1997Paper

Sequence and drug susceptibility of subtype C reverse transcriptase from human immunodeficiency virus type 1 seroconverters in Zimbabwe

Journal of Virology
R W ShaferD A Katzenstein

Abstract

Naturally occurring human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) variability has implications for the success of antiretroviral therapy. We determined the sequence of the polymerase-coding region of RT from virus isolates from 12 Zimbabwean individuals recently infected with HIV-1. The 12 RT sequences differed from the consensus B RT sequence at 10.5% of nucleotides and 5.8% of amino acids. Susceptibility testing of five isolates to zidovudine, didanosine, lamivudine, and nevirapine demonstrated susceptibilities similar to those of wild-type subtype B isolates. Phylogenetic analysis of 40 HIV-1 RT sequences, including the 12 Zimbabwean subtype C sequences, 11 subtype B sequences, and the 17 remaining published non-subtype B sequences showed sufficient intrasubtype RT sequence variation to differentiate subtype A, B, C, and D isolates. Five recently reported subtype C RT sequences from India grouped with the Zimbabwean subtype C sequences but had significantly less intraisolate sequence variation. Both intra- and intersubtype RT comparisons were notable for extraordinarily high ratios of synonymous to nonsynonymous differences. Although substitutions in the HIV-1 RT gene are limited by functional constr...Continue Reading

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Citations

Apr 20, 2000·AIDS Research and Human Retroviruses·A HolguínV Soriano
Jul 13, 2000·AIDS Research and Human Retroviruses·M BatraD A Katzenstein
Jun 8, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·P J WeidleE Lackritz
Mar 5, 2004·The New England Journal of Medicine·François Clavel, Allan J Hance
Nov 8, 2008·PLoS Computational Biology·Osnat PennTal Pupko
Feb 15, 2001·AIDS Research and Human Retroviruses·N KochC Tamalet
Aug 22, 2006·The Journal of Infectious Diseases·David Katzenstein
May 31, 2002·AIDS Research and Human Retroviruses·Candice PillayLynn Morris
Jan 14, 2005·Journal of Medical Virology·Africa HolguínVincent Soriano
Oct 13, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·L M SkinnerM Katzman
May 14, 2005·Antiviral Chemistry & Chemotherapy·Hermann BussmannMax Essex
Apr 5, 2002·Clinical Microbiology Reviews·Robert W Shafer
May 29, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·M PetrellaM A Wainberg

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