Sequence and Expression Analysis of Interferon Regulatory Factor 10 (IRF10) in Three Diverse Teleost Fish Reveals Its Role in Antiviral Defense

PloS One
Qiao-Qing XuTiehui Wang

Abstract

Interferon regulatory factor (IRF) 10 was first found in birds and is present in the genome of other tetrapods (but not humans and mice), as well as in teleost fish. The functional role of IRF10 in vertebrate immunity is relatively unknown compared to IRF1-9. The target of this research was to clone and characterize the IRF10 genes in three economically important fish species that will facilitate future evaluation of this molecule in fish innate and adaptive immunity. In the present study, a single IRF10 gene was cloned in grass carp Ctenopharyngodon idella and Asian swamp eel Monopterus albus, and two, named IRF10a and IRF10b, in rainbow trout Oncorhynchus mykiss. The fish IRF10 molecules share highest identities to other vertebrate IRF10s, and have a well conserved DNA binding domain, IRF-associated domain, and an 8 exon/7 intron structure with conserved intron phase. The presence of an upstream ATG or open reading frame (ORF) in the 5'-untranslated region of different fish IRF10 cDNA sequences suggests potential regulation at the translational level, and this has been verified by in vitro transcription/translation experiments of the trout IRF10a cDNA, but would still need to be validated in fish cells. Both trout IRF10 paral...Continue Reading

References

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Citations

Feb 12, 2021·Genes·Thomas C ClarkBertrand Collet

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Datasets Mentioned

BETA
AB359170
HG917960
Q90WI0

Methods Mentioned

BETA
PCR
FCS

Software Mentioned

SPSS
MatGAT
CLUSTALW
AlignIR
ExPASy
BOXSHADE
BLAST ( Basic Local Alignment Search Tool )
MEGA6
Spidey
Ensembl

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