Sequence and expression analysis of two T helper master transcription factors, T-bet and GATA3, in rainbow trout Oncorhynchus mykiss and analysis of their expression during bacterial and parasitic infection

Fish & Shellfish Immunology
Tiehui WangC J Secombes

Abstract

The polarization of naïve CD4+ T cells to T helper (Th)1 or Th2 cells is specified by two master transcription factors, T-bet and GATA3, and is an essential feature of mammalian adaptive immune responses to pathogens and the development of long-lasting immunity. We report here the cloning of rainbow trout Oncorhynchus mykiss T-bet and GATA3, to allow the future evaluation of the existence of Th1 and Th2 cells in salmonid fish. The trout T-bet translation shares high amino acid identities to other fish T-bet molecules (71-72%) but low identities to mammalian T-bet genes (41-42%), although the middle T-box DNA binding domain is highly conserved among all the T-bet proteins from fish and mammals. The trout GATA3 has high amino acid sequence identities (73-88%) to all known vertebrate molecules, with two highly conserved zinc finger motifs. The identity of the trout T-bet and GATA3 molecules was confirmed by phylogenetic tree analysis. A comparable expression level of T-bet and GATA3 was seen in the spleen, head kidney and muscle in healthy trout, but a higher expression level of GATA3 was seen in the gills, brain, skin and intestine relative to that of T-bet. T-bet and GATA3 expression was modulated by different stimulants. The T ...Continue Reading

Citations

May 28, 2013·Developmental and Comparative Immunology·Giuseppe Scapigliati
May 15, 2013·Fish & Shellfish Immunology·Uwe FischerTeruyuki Nakanishi
Sep 17, 2013·Fish & Shellfish Immunology·Tiehui Wang, Christopher J Secombes
Nov 19, 2013·Fish & Shellfish Immunology·Fumio TakizawaUwe Fischer
Apr 9, 2013·PloS One·Rosario CastroPierre Boudinot
Aug 22, 2013·Developmental and Comparative Immunology·Hetron Mweemba Munang'anduØystein Evensen
Dec 29, 2011·Journal of Signal Transduction·Tiehui WangChristopher J Secombes
Feb 26, 2016·Developmental and Comparative Immunology·Carolina TafallaUwe Fischer
Oct 3, 2012·Fish & Shellfish Immunology·Eve MartinJean-Pol Frippiat
Jun 15, 2011·Fish & Shellfish Immunology·Tiehui WangChristopher J Secombes
May 25, 2011·Developmental and Comparative Immunology·C J SecombesS Bird
Mar 19, 2011·Developmental and Comparative Immunology·Kerry J Laing, John D Hansen
Sep 18, 2015·Frontiers in Immunology·Hetron Mweemba Munang'anduØystein Evensen
Jun 13, 2015·Journal of Immunology Research·Hetron Mweemba Munang'andu, Øystein Evensen
Jan 30, 2015·Veterinary Immunology and Immunopathology·A Rivas-AravenaA M Sandino
Aug 20, 2016·Fish & Shellfish Immunology·Sohye YoonChristopher J Secombes
Jun 29, 2017·Developmental and Comparative Immunology·Ida Bergva AasErling Olaf Koppang
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Jan 9, 2019·Veterinary Research·Hassan AshfaqMansour El-Matbouli
May 14, 2020·Parasite Immunology·Christyn BaileyCarolina Tafalla
Dec 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tiehui WangChristopher J Secombes
Apr 8, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin MaiseyMónica Imarai

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