PMID: 9634146Jun 20, 1998Paper

Sequence and expression of glutamic acid decarboxylase isoforms in the developing zebrafish

The Journal of Comparative Neurology
S C MartinJ H Sandell

Abstract

We describe the isolation two glutamic acid decarboxylase (GAD) cDNAs from zebrafish with over 84% identity to human GAD65 and GAD67. In situ hybridization studies revealed that both GAD65 and GAD67 were expressed in the early zebrafish embryo during the period of axonogenesis, suggesting a role for GABA prior to synapse formation. Both GAD genes were detected in the telencephalon, in the nucleus of the medial longitudinal fasciculus in the midbrain, and at the border regions of the rhombomeres in the rostral hindbrain. In the caudal hindbrain, only GAD67 was detected (in neurons with large-caliber axons). In the spinal cord, both GAD genes were detected in dorsal longitudinal neurons, commissural secondary ascending neurons, ventral longitudinal neurons, and Kolmer-Agduhr neurons. Immunohistochemistry for gamma-aminobutyric acid (GABA) revealed that GABA is produced at all sites of GAD expression, including the novel cells in the caudal hindbrain. These results are discussed in the context of the hindbrain circuitry that supports the escape response. We conclude that fish, like mammals, have two GAD genes. The zebrafish GAD65 and GAD67 are present in identified neurons in the forebrain, midbrain, hindbrain, and spinal cord, an...Continue Reading

Citations

Mar 24, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Katharine E Lewis
Jun 26, 2009·Development·Nikolas NikolaouDavid G Wilkinson
Jul 17, 2003·The Journal of Comparative Neurology·Miguel Meléndez-FerroMaría Celina Rodicio
Mar 14, 2008·Journal of Pharmacological and Toxicological Methods·Matthew J WinterThomas H Hutchinson
Mar 12, 2008·Brain Research Bulletin·Iván CarreraIsabel Rodríguez-Moldes
Apr 30, 2013·Environmental Toxicology and Chemistry·David C BencicGerald T Ankley
Jul 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ryan B MacDonaldMarc Ekker
Nov 26, 2014·The Journal of Comparative Neurology·Talia Levitas-DjerbiLior Appelbaum
Aug 12, 2014·Congenital Anomalies·Yuhei NishimuraToshio Tanaka
Apr 18, 2009·Developmental Biology·Young-Ki BaeMasahiko Hibi
Nov 1, 2005·Developmental Biology·Ayumi MiyakeNobuyuki Itoh
Jul 11, 2006·Developmental Biology·Birgit AdolfLaure Bally-Cuif
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Apr 16, 2002·The Journal of Comparative Neurology·Miguel Meléndez-FerroRamón Anadón
Mar 23, 2017·Endocrinology·Yanlong SongWei Hu

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