Sequence and expression of the Drosophila phenylalanine hydroxylase mRNA

Gene
G MoralesC Alonso

Abstract

We report the cloning, nucleotide (nt) sequence and expression of the cDNA (pah) encoding phenylalanine hydroxylase (PAH) of Drosophila melanogaster. The strong hybridization signals observed in genomic blots when D. melanogaster DNA was probed with 32P-labeled human pah cDNA, indicated the existence of a high degree of sequence similarity between the pah genes of both species. The length of the pah genomic fragment is about 30 to 40 kb. The cDNA contains 84 bp of the 5'-untranslated region, 1359 bp of the protein-coding region and 87 bp of the 3' region, with only one polyadenylation signal. The isolated cDNA is probably full-length, since the size of the D. melanogaster PAH mRNA is 1.5 kb. At the nt level, the similarity of the D. melanogaster cDNA with human and rat pah cDNAs is 57.9% and 58.1%, respectively. The highest similarities are restricted to the nt sequence coding for the presumed hydroxylation domain. There is no nt sequence similarity between the first three exons of the human pah gene and an equivalent fraction of the D. melanogaster pah gene. At the amino acid (aa) level, the similarity in the presumed hydroxylation domain is 88.5%, in which two motifs of the structure AGLLSSXXXL are found, where X represents a...Continue Reading

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Citations

May 1, 1991·Somatic Cell and Molecular Genetics·M DasovichS L Woo
Mar 1, 2003·Insect Biochemistry and Molecular Biology·J K JohnsonB M Christensen
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A Jimenez-RuizC Alonso
Oct 19, 2011·Archives of Biochemistry and Biophysics·Paul F Fitzpatrick
Apr 12, 2005·Archives of Insect Biochemistry and Physiology·Chandra M Coleman, Wendi S Neckameyer
Nov 17, 2004·Insect Biochemistry and Molecular Biology·Li-Chun InfangerBruce M Christensen
Aug 5, 2008·Science in China. Series C, Life Sciences·Qin WangChangxin Wu
Nov 7, 1996·Chemical Reviews·T. Joseph Kappock, John P. Caradonna

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