Sequence and organization of pMAC, an Acinetobacter baumannii plasmid harboring genes involved in organic peroxide resistance

Plasmid
Caleb W DorseyLuis A Actis

Abstract

Acinetobacter baumannii 19606 harbors pMAC, a 9540-bp plasmid that contains 11 predicted open-reading frames (ORFs). Cloning and transformation experiments using Acinetobacter calcoaceticus BD413 mapped replication functions within a region containing four 21-bp direct repeats (ori) and ORF 1, which codes for a predicted replication protein. Subcloning and tri-parental mating experiments mapped mobilization functions to the product of ORF 11 and an adjacent predicted oriT. Three ORFs code for proteins that share similarity to hypothetical proteins encoded by plasmid genes found in other bacteria, while the predicted products of three others do not match any known sequence. The product of ORF 8 is similar to Ohr, a hydroperoxide reductase responsible for organic peroxide detoxification and resistance in bacteria. This ORF is immediately upstream of a coding region whose product is related to the MarR family of transcriptional regulators. Disk diffusion assays showed that A. baumannii 19606 is resistant to the organic peroxide-generating compounds cumene hydroperoxide (CHP) and tert-butyl hydroperoxide (t-BHP), although to levels lower than those detected in Pseudomonas aeruginosa PAO1. Cloning and introduction of the ohr and mar...Continue Reading

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Citations

Aug 30, 2008·Antimicrobial Agents and Chemotherapy·Raffaele ZarrilliMaria Triassi
Jul 28, 2010·Antimicrobial Agents and Chemotherapy·Alessia BertiniAlessandra Carattoli
Apr 18, 2007·Antimicrobial Agents and Chemotherapy·Alessia BertiniAlessandra Carattoli
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Sep 2, 2017·Frontiers in Microbiology·Soo Sum Lean, Chew Chieng Yeo
Jul 14, 2020·World Journal of Microbiology & Biotechnology·Marco A BrovedanGuillermo D Repizo

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