Sequence and phylogenetic analyses of the structural genes of virulent isolates and vaccine strains of peste des petits ruminants virus from India

Transboundary and Emerging Diseases
V BalamuruganR K Singh

Abstract

Peste des petits ruminants (PPR) is an acute, highly contagious, notifiable and economically important transboundary viral disease of sheep and goats. In this study, sequence and phylogenetic analyses of structural protein genes, namely the nucleocapsid (N), the matrix (M), the fusion (F) and the haemagglutinin (H) coding sequences of virulent and vaccine strains of PPR virus (PPRV), were undertaken to determine the genetic variations between field isolates and vaccine strains. The open reading frame (ORF) of these genes of the isolates/strains was amplified by RT-PCR, cloned and sequenced. The ORF of N, M, F and H genes was 1578, 1008, 1641 and 1830 nucleotides (nt) in length and encodes polypeptides of 525, 335, 546 and 609 amino acids (aa), respectively, as reported earlier. Comparative sequence analyses of these four genes of isolates/strains were carried out with published sequences. It revealed an identity of 97.7-100% and 97.7-99.8% among the Asian lineage IV and 89.6-98.7% and 89.8-98.9% with other lineages of PPRV at nt and aa levels, respectively. The phylogenetic analyses of these isolates based on the aa sequences showed that all the viruses belonged to lineage IV along with other Asian isolates. This is in agreemen...Continue Reading

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Citations

Nov 23, 2011·Tropical Animal Health and Production·Vinayagamurthy BalamuruganKrishnamsetty Prabhudas
Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·A K SanthoshShrikrishna Isloor
Jan 18, 2011·Transboundary and Emerging Diseases·Y S MalikS Chakravarti
Jan 1, 2012·Uirusu·Fumio Seki, Makoto Takeda
Sep 17, 2014·Veterinary Research Communications·Fuxiao LiuZhiliang Wang
Mar 15, 2016·The Onderstepoort Journal of Veterinary Research·Timothy Y WomaMelvyn Quan
Apr 5, 2016·Current Microbiology·Barkha RattaBhaskar Sharma
Sep 25, 2012·Journal of Veterinary Science·Vinayagamurthy BalamuruganRaj Kumar Singh
Jun 19, 2012·Journal of Veterinary Science·Vinayagamurthy BalamuruganRaj Kumar Singh
Jul 19, 2019·Molecular Biology Reports·Aziz-Ul- RahmanMuhammad Zubair Shabbir
Jul 28, 2018·Transboundary and Emerging Diseases·Muhammad Zubair ShabbirMuhammad Munir

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