Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria

Virusdisease
O A Fagbohun, T O Omobowale

Abstract

Canine parvovirus-2 (CPV-2) is the most common viral cause of gastroenteritis in puppies in Nigeria and worldwide. After its emergence in 1978, the wild-type CPV-2 was rapidly replaced by three antigenic subtypes CPV-2a, CPV-2b and CPV-2c. Subtype CPV-2a has been reported in Nigeria based on limited number of samples. As such, this study was carried out with 56 faecal samples and four CPV-2 vaccines using polymerase chain reaction and sequencing to have a better idea of CPV-2 subtypes circulating in dogs in Nigeria. 54 (96.4%) out of the 56 samples were positive for CPV-2 subtypes. CPV-2a is the predominant subtype followed by CPV-2b and CPV-2c. Also, co-existence of subtypes CPV-2a and CPV-2b; and CPV-2a and CPV-2c were found in some dogs. Sequence analysis revealed the occurrence of three mutations D413 N, N426D/E, and T440A. To our knowledge, this is the first report of the three subtypes of CPV-2 in Nigeria.

References

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Citations

Oct 4, 2019·Transboundary and Emerging Diseases·Kenneth Ikejiofor OgbuAnnalisa Guercio
Apr 8, 2020·Veterinary World·N InthongT Sirinarumitr
Nov 28, 2020·Comparative Immunology, Microbiology and Infectious Diseases·Kenneth Ikejiofor OgbuBoniface Maduka Anene
Jul 12, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Matthew Terzungwe TionGregory Anatoliiovych Zon

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Datasets Mentioned

BETA
KX192421
KX192419
GU139566
MG583676
FJ005261
KT074338

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
MEGA

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