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Sequence and primer independent stochastic heterogeneity in PCR amplification efficiency revealed by single molecule barcoding

bioRxiv

Nov 13, 2014

Katharine BestBenny M Chain

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Abstract

The polymerase chain reaction (PCR) is one of the most widely used techniques in molecular biology. In combination with High Throughput Sequencing (HTS), PCR is widely used to quantify transcript abundance for RNA-seq and especially in the context of analysis of T cell and B cell recept...read more

Mentioned in this Paper

Small Molecule
Polymerase Chain Reaction
Oligonucleotide Primers
Study
Simulation
DNA Amplification
B-Lymphocytes
DNA Barcoding, Taxonomic
Receptors, Antigen, B-Cell
T-Lymphocyte
5
2
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  • Sequence and primer independent stochastic heterogeneity in PCR amplification efficiency revealed by single molecule barcoding

    bioRxiv

    Nov 13, 2014

    Katharine BestBenny M Chain

    PMID: 990011411

    DOI: 10.1101/011411

    Abstract

    The polymerase chain reaction (PCR) is one of the most widely used techniques in molecular biology. In combination with High Throughput Sequencing (HTS), PCR is widely used to quantify transcript abundance for RNA-seq and especially in the context of analysis of T cell and B cell recept...read more

    Mentioned in this Paper

    Small Molecule
    Polymerase Chain Reaction
    Oligonucleotide Primers
    Study
    Simulation
    5
    2

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