Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA

The EMBO Journal
Z Kiss-LászlóT Kiss

Abstract

Site-specific 2'-O-ribose methylation of eukaryotic rRNAs is guided by small nucleolar RNAs (snoRNAs). The methylation guide snoRNAs carry long perfect complementaries to rRNAs. These antisense elements are located either in the 5' half or in the 3' end region of the snoRNA, and are followed by the conserved D' or D box motifs, respectively. An uninterrupted helix formed between the rRNA and the antisense element of the snoRNA, in conjunction with the adjacent D' or D box, constitute the recognition signal for the putative methyltransferase. Here, we have identified an additional essential box element common to methylation guide snoRNAs, termed the C' box. We show that the C' box functions in concert with the D' box and plays a crucial role in the methyltransfer reaction directed by the upstream antisense element and the D' box. We also show that an internal fragment of U24 methylation guide snoRNA, encompassing the upstream antisense element and the D' and C' box motifs, can support the site-specific methylation of rRNA. This strongly suggests that the C box of methylation guide snoRNAs plays an essential role in the methyltransfer reaction guided by the 3'-terminal antisense element and the D box of the snoRNA.

References

Jun 1, 1992·Genes & Development·S J BasergaX W Yang
Dec 21, 1981·Nucleic Acids Research·G M VeldmanJ P Ebel
Jan 1, 1995·Annual Review of Biochemistry·E S Maxwell, M J Fournier
Jul 1, 1995·Trends in Biochemical Sciences·J P BachellerieM J Fournier
Jun 1, 1995·Genes & Development·T Kiss, W Filipowicz
Dec 1, 1995·Yeast·J Venema, D Tollervey
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J OfengandB G Lane
Oct 24, 1996·Nature·T Maden
Nov 1, 1996·Current Biology : CB·B A Peculis, S M Mount
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·K T TycowskiJ A Steitz
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·R K Mishra, G L Eliceiri
Jun 1, 1997·Current Opinion in Cell Biology·D Tollervey, T Kiss
May 30, 1997·Cell·C M Smith, J A Steitz
Jul 1, 1997·Trends in Biochemical Sciences·J P Bachellerie, J Cavaillé
Aug 1, 1997·Current Biology : CB·B Peculis
Feb 1, 1959·Biochimica Et Biophysica Acta·J D SMITH, D B DUNN

❮ Previous
Next ❯

Citations

Nov 30, 2002·Biochimie·Jean Pierre BachellerieAlexander Hüttenhofer
Jul 18, 2002·Gene·Irina K GogolevskayaDmitri A Kramerov
Apr 1, 2000·RNA·D FilippiniE Caffarelli
Sep 27, 2007·ACS Chemical Biology·Christine S ChowSantosh K Mahto
Feb 27, 2010·Journal of the American Chemical Society·Stefan JehleTeresa Carlomagno
Oct 15, 2013·Nature·Audrone LapinaiteTeresa Carlomagno
Sep 28, 2013·Physical Chemistry Chemical Physics : PCCP·Wei YeHai-Feng Chen
Nov 17, 1999·Molecular Biology of the Cell·T S LangeS A Gerbi
Jul 9, 1999·Molecular Biology of the Cell·A NarayananM P Terns
Dec 8, 1998·The EMBO Journal·A HenrasM Caizergues-Ferrer
Dec 10, 1998·Nucleic Acids Research·D A Samarsky, M J Fournier
Nov 7, 2003·Nucleic Acids Research·Marie-Line BortolinBéatrice Clouet-d'Orval
Aug 31, 2004·Nucleic Acids Research·Anders AspegrenFredrik Söderbom
Jun 4, 2005·Nucleic Acids Research·Bruno CharpentierChristiane Branlant
Feb 8, 2007·Nucleic Acids Research·Steve L ReichowGabriele Varani
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S A GerbiT S Lange
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·T KissM Weber
Sep 12, 2009·Science·Franziska BleichertSusan J Baserga
Mar 25, 2000·Molecular and Cellular Biology·D L Lafontaine, D Tollervey
Mar 31, 2005·Molecular and Cellular Biology·Ghada GhazalSherif Abou Elela
Jan 22, 2008·Biochemistry. Biokhimii︠a︡·J A Makarova, D A Kramerov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.