Sequence and tissue-dependent RNA expression of mouse FAD-linked glycerol-3-phosphate dehydrogenase

Archives of Biochemistry and Biophysics
R A KozaL P Kozak

Abstract

A 2432-bp cDNA for mouse FAD-linked glycerol-3-phosphate dehydrogenase, a nuclear-encoded enzyme associated with the inner mitochondrial membrane, was isolated from a Lambda ZAP phage library generated from brown adipocyte mRNA. The amino acid sequence was 95 and 93% homologous to the rat and human enzymes. PvuII and SacI polymorphisms between Mus spretus and C57BL/6J were used to map the mouse FAD-linked glycerol-3-phosphate dehydrogenase gene (Gdm1) to chromosome 2, 33 cM from the centromere. Northern blot analysis showed that brown adipose tissue predominantly expressed a 6.5-kb mRNA with lower expression of 4.5- and 2.4-kb forms, whereas brain and pancreatic islets almost exclusively expressed a 6.5-kb transcript, muscle expressed a 4.5-kb transcript, and testis expressed a 2.4-kb RNA form. Analysis of poly(A)+ RNA from brown adipose tissue suggested that all RNA forms are polyadenylated. Among the tissues examined, FAD-linked glycerol-3-phosphate dehydrogenase protein levels and enzyme activity were highest in brown adipose tissue, and a consistent correlation between protein levels and enzyme activity in all tissues was observed. RNA levels corresponded to protein levels in all tissues except testis, where high levels of ...Continue Reading

Citations

May 15, 2010·The Journal of Biological Chemistry·Rebecca OelkrugMartin Jastroch
Nov 6, 2012·The Journal of Biological Chemistry·Adam L OrrMartin D Brand
Dec 12, 2012·Biochimica Et Biophysica Acta·Tomáš MráčekJosef Houštěk
Aug 16, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anthony A RubertoKenneth B Storey
Sep 30, 2017·Molecular Human Reproduction·Luís CrisóstomoPedro F Oliveira
Mar 20, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Assim AlfaddaJ Enrique Silva
Nov 12, 2005·Bioscience Reports·J Enrique Silva
Apr 8, 2006·Physiological Reviews·J Enrique Silva
Oct 21, 2017·Nature Reviews. Endocrinology·Matthias J Betz, Sven Enerbäck
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Aug 7, 2019·Nature Immunology·P Kent LangstonTiffany Horng
Aug 24, 2000·The Journal of Biological Chemistry·Q GongM J MacDonald
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·A Yu AndreyevA A Starkov
Nov 21, 2001·The Journal of Biological Chemistry·Eulàlia MontellAnna M Gómez-Foix
Jun 6, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Monika CahovaLudmila Kazdova
Sep 9, 2020·Endocrine Reviews·Traci E LaMoia, Gerald I Shulman
Jul 25, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·W BerradaN Bourhim
Aug 6, 2017·Progress in Lipid Research·Lu-Lu XueJian-Guo Jiang
Oct 28, 2008·Archives of Biochemistry and Biophysics·Tomás MrácekJosef Houstek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.