Sequence-based autosomal STR characterization in four US populations using PowerSeq™ Auto/Y system.

Forensic Science International. Genetics
Deborah S B S SilvaSeth A Faith

Abstract

The forensic science community is poised to utilize modern advances in massively parallel sequencing (MPS) technologies to better characterize biological samples with higher resolution. A critical component towards the advancement of forensic DNA analysis with these technologies is a comprehensive understanding of the diversity and population distribution of sequence-based short tandem repeat (STR) alleles. Here we analyzed 786 samples of individuals from different population groups, including four of the mostly commonly encountered in forensic casework in the USA. DNA samples were amplified with the PowerSeq™ Auto/Y System Prototype Kit (Promega Corp.), and sequencing was performed on an Illumina® MiSeq instrument. Sequence data were analyzed using a bioinformatics processing tool, Altius. For additional data analysis and profile comparison, capillary electrophoresis (CE) size-based STR genotypes were generated for a subset of individuals, and where possible, also with a second commercially available MPS STR assay. Autosomal STR loci were analyzed and frequencies were calculated based on sequence composition. Also, population genetics studies were performed, with Hardy-Weinberg equilibrium, polymorphic information content (PIC)...Continue Reading

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Feb 24, 2015·Forensic Science International. Genetics·Claus Børsting, Niels Morling
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Jul 5, 2017·Forensic Science International. Genetics·Leonor GusmãoAngel Carracedo
Jul 26, 2017·Forensic Science International. Genetics·Alexandre Gouy, Martin Zieger
Aug 25, 2017·Forensic Science International. Genetics·Sarah F BaileySeth A Faith
Aug 26, 2018·Forensic Science International. Genetics·Katherine Butler GettingsPeter M Vallone

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Citations

Aug 27, 2021·International Journal of Legal Medicine·Petra Hölzl-MüllerWalther Parson

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