Sequence-based characterization of  Listeria monocytogenes  strains isolated from domestic retail meat in the Tokyo metropolitan area of Japan

Japanese Journal of Infectious Diseases
Yuko YoshikawaFukiko Ueda

Abstract

To assess the level of Listeria monocytogenes contamination of domestic retail meat in Tokyo, Japan, we compared isolates from 2004 to 2007 with those isolated before 2003. The overall prevalence of L. monocytogenes among these samples significantly diminished over time (1998-2003, 28.0%; 2004-2007, 17.6%) reflecting a significant decrease in the frequency of contamination of beef. Serotype 1/2a was isolated most frequently, reflecting a change in the predominant serotype in pork from 1/2c to 1/2a. We performed a simple genetic subtyping method based on three genes, iap, sigB, and actA, as well as traditional multilocus sequence typing to classify the allele types (ATs). No extensive variation among sequence types was detected; however, increased genetic diversity among the ATs of the three genes in the 2004-2007 isolates was evident. We identified AT 26 of the iap gene, not previously reported in Japanese isolates, and six ATs of the sigB gene, including four with nonsense mutations not currently registered in L. monocytogenes DNA databases. sigB is an evolutionally conserved gene that plays a role in the stress response. Our results indicate that the sigB gene may be relatively unstable among L. monocytogenes strains circulat...Continue Reading

References

Feb 8, 2003·Journal of Clinical Microbiology·C SalcedoJ A Vázquez
Aug 11, 2005·International Journal of Food Microbiology·Fukiko UedaRyo Hondo
Aug 28, 2007·Microbes and Infection·Bala Swaminathan, Peter Gerner-Smidt
Nov 7, 2007·The Journal of Veterinary Medical Science·Tomoko TakahashiFukiko Ueda
Sep 6, 2008·PLoS Pathogens·Marie RagonSylvain Brisse
Sep 11, 2010·Journal of Food Protection·Yoshitsugu OchiaiFukiko Ueda
Feb 1, 2011·International Journal of Food Microbiology·Brigitte Carpentier, Olivier Cerf
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Oct 12, 2016·The Journal of Veterinary Medical Science·Yuko YoshikawaFukiko Ueda

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